[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
Gene silencing through CRISPR interference in bacteria: current advances and future prospects
R Zhang, W Xu, S Shao, Q Wang - Frontiers in Microbiology, 2021 - frontiersin.org
Functional genetic screening is an important method that has been widely used to explore
the biological processes and functional annotation of genetic elements. CRISPR/Cas …
the biological processes and functional annotation of genetic elements. CRISPR/Cas …
The impact of genetic diversity on gene essentiality within the Escherichia coli species
Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …
the set of genes shared by all strains, known as the core genome, represents about half the …
Protein engineering and iterative multimodule optimization for vitamin B6 production in Escherichia coli
L Liu, J Li, Y Gai, Z Tian, Y Wang, T Wang, P Liu… - Nature …, 2023 - nature.com
Vitamin B6 is an essential nutrient with extensive applications in the medicine, food, animal
feed, and cosmetics industries. Pyridoxine (PN), the most common commercial form of …
feed, and cosmetics industries. Pyridoxine (PN), the most common commercial form of …
Metabolism of non-growing bacteria
A main function of bacterial metabolism is to supply biomass building blocks and energy for
growth. This seems to imply that metabolism is idle in non-growing bacteria. But how …
growth. This seems to imply that metabolism is idle in non-growing bacteria. But how …
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces
The rise of antibiotic-resistant bacteria represents a major threat to global health, creating an
urgent need to discover new antibiotics. Natural products derived from the genus …
urgent need to discover new antibiotics. Natural products derived from the genus …
Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a
CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene
regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT …
regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT …
Map** temperature‐sensitive mutations at a genome scale to engineer growth switches in Escherichia coli
Temperature‐sensitive (TS) mutants are a unique tool to perturb and engineer cellular
systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each …
systems. Here, we constructed a CRISPR library with 15,120 Escherichia coli mutants, each …
[HTML][HTML] Optimized genome-wide CRISPR screening enables rapid engineering of growth-based phenotypes in Yarrowia lipolytica
CRISPR-Cas9 functional genomic screens uncover gene targets linked to various
phenotypes for metabolic engineering with remarkable efficiency. However, these genome …
phenotypes for metabolic engineering with remarkable efficiency. However, these genome …
Design of microbial catalysts for two-stage processes
Microbial catalysts must partition incoming substrate between the synthesis of biomass and
the synthesis of a desired product. Although biomass synthesis generates more catalyst and …
the synthesis of a desired product. Although biomass synthesis generates more catalyst and …