Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

Abstract The 4D Nucleome Network aims to develop and apply approaches to map the
structure and dynamics of the human and mouse genomes in space and time with the goal …

How enhancers control target gene expression over long genomic distances remains an
important unsolved problem. Here we investigated enhancer–promoter communication by …

It remains unclear why acute depletion of CTCF (CCCTC-binding factor) and cohesin only
marginally affects expression of most genes despite substantially perturbing three …

Eukaryotic genomes are organized into chromatin domains. The molecular mechanisms
driving the formation of these domains are difficult to dissect in vivo and remain poorly …

Whereas folding of genomes at the large scale of epigenomic compartments and
topologically associating domains (TADs) is now relatively well understood, how chromatin …

Over the past decade, 3C-related methods have provided remarkable insights into
chromosome folding in vivo. To overcome the limited resolution of prior studies, we extend a …

In higher eukaryotes, many genes are regulated by enhancers that are 104–106 base pairs
(bp) away from the promoter. Enhancers contain transcription-factor-binding sites (which are …

Chromosome conformation capture (3C) assays are used to map chromatin interactions
genome-wide. Chromatin interaction maps provide insights into the spatial organization of …

Mammalian genomes are folded into topologically associating domains (TADs), consisting
of chromatin loops anchored by CTCF and cohesin. Some loops are cell-type specific. Here …