The molecular basis for cellular function of intrinsically disordered protein regions
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …
Principles governing the phase separation of multidomain proteins
A variety of membraneless organelles, often termed “biological condensates”, play an
important role in the regulation of cellular processes such as gene transcription, translation …
important role in the regulation of cellular processes such as gene transcription, translation …
A synergy between site-specific and transient interactions drives the phase separation of a disordered, low-complexity domain
TAR DNA-binding protein 43 (TDP-43) is involved in key processes in RNA metabolism and
is frequently implicated in many neurodegenerative diseases, including amyotrophic lateral …
is frequently implicated in many neurodegenerative diseases, including amyotrophic lateral …
Driving forces behind phase separation of the carboxy-terminal domain of RNA polymerase II
D Flores-Solis, IP Lushpinskaia, AA Polyansky… - Nature …, 2023 - nature.com
Eukaryotic gene regulation and pre-mRNA transcription depend on the carboxy-terminal
domain (CTD) of RNA polymerase (Pol) II. Due to its highly repetitive, intrinsically disordered …
domain (CTD) of RNA polymerase (Pol) II. Due to its highly repetitive, intrinsically disordered …
Expanding the molecular language of protein liquid–liquid phase separation
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …
has important implications for analysing cellular function, treating disease and designing …
Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
Determinants that enable disordered protein assembly into discrete condensed phases
Cells harbour numerous mesoscale membraneless compartments that house specific
biochemical processes and perform distinct cellular functions. These protein-and RNA-rich …
biochemical processes and perform distinct cellular functions. These protein-and RNA-rich …
A solid beta-sheet structure is formed at the surface of FUS droplets during aging
Phase transitions are important to understand cell dynamics, and the maturation of liquid
droplets is relevant to neurodegenerative disorders. We combined NMR and Raman …
droplets is relevant to neurodegenerative disorders. We combined NMR and Raman …
Self-assembly of promoter DNA and RNA Pol II machinery into transcriptionally active biomolecular condensates
Transcription in the nucleus occurs in a concentrated, dense environment, and no
reasonable biochemical facsimile of this milieu exists. Such a biochemical environment …
reasonable biochemical facsimile of this milieu exists. Such a biochemical environment …
Role of strong localized vs weak distributed interactions in disordered protein phase separation
Interaction strength and localization are critical parameters controlling the single-chain and
condensed-state properties of intrinsically disordered proteins (IDPs). Here, we decipher …
condensed-state properties of intrinsically disordered proteins (IDPs). Here, we decipher …