Control of protein stability by post-translational modifications

JM Lee, HM Hammarén, MM Savitski… - Nature …, 2023 - nature.com
Post-translational modifications (PTMs) can occur on specific amino acids localized within
regulatory domains of target proteins, which control a protein's stability. These regions …

Technical challenges of working with extracellular vesicles

MI Ramirez, MG Amorim, C Gadelha, I Milic, JA Welsh… - Nanoscale, 2018 - pubs.rsc.org
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …

Direct observation of glycans bonded to proteins and lipids at the single-molecule level

K Anggara, L Sršan, T Jaroentomeechai, X Wu… - Science, 2023 - science.org
Proteins and lipids decorated with glycans are found throughout biological entities, playing
roles in biological functions and dysfunctions. Current analytical strategies for these glycan …

Multiscale simulations of biological membranes: the challenge to understand biological phenomena in a living substance

G Enkavi, M Javanainen, W Kulig, T Róg… - Chemical …, 2019 - ACS Publications
Biological membranes are tricky to investigate. They are complex in terms of molecular
composition and structure, functional over a wide range of time scales, and characterized by …

The growing landscape of lysine acetylation links metabolism and cell signalling

C Choudhary, BT Weinert, Y Nishida, E Verdin… - … reviews Molecular cell …, 2014 - nature.com
Lysine acetylation is a conserved protein post-translational modification that links acetyl-
coenzyme A metabolism and cellular signalling. Recent advances in the identification and …

Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Lysine acetylation targets protein complexes and co-regulates major cellular functions

C Choudhary, C Kumar, F Gnad, ML Nielsen… - Science, 2009 - science.org
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key
role in regulating gene expression. Technological limitations have so far prevented a global …

[HTML][HTML] Precision map** of an in vivo N-glycoproteome reveals rigid topological and sequence constraints

DF Zielinska, F Gnad, JR Wiśniewski, M Mann - Cell, 2010 - cell.com
N-linked glycosylation is a biologically important protein modification, but only a small
fraction of modification sites have been mapped. We developed a" filter aided sample …

Chemical glycoproteomics

KK Palaniappan, CR Bertozzi - Chemical reviews, 2016 - ACS Publications
Chemical tools have accelerated progress in glycoscience, reducing experimental barriers
to studying protein glycosylation, the most widespread and complex form of posttranslational …

[HTML][HTML] Proteomics: challenges, techniques and possibilities to overcome biological sample complexity

K Chandramouli, PY Qian - Human genomics and proteomics: HGP, 2009 - ncbi.nlm.nih.gov
Proteomics is the large-scale study of the structure and function of proteins in complex
biological sample. Such an approach has the potential value to understand the complex …