High-throughput determination of RNA structures

EJ Strobel, AM Yu, JB Lucks - Nature Reviews Genetics, 2018 - nature.com
RNA performs and regulates a diverse range of cellular processes, with new functional roles
being uncovered at a rapid pace. Interest is growing in how these functions are linked to …

Advances in RNA 3D structure modeling using experimental data

B Li, Y Cao, E Westhof, Z Miao - Frontiers in genetics, 2020 - frontiersin.org
RNA is a unique bio-macromolecule that can both record genetic information and perform
biological functions in a variety of molecular processes, including transcription, splicing …

[HTML][HTML] The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif

C Schaeffer, B Bardoni, JL Mandel, B Ehresmann… - The EMBO …, 2001 - embopress.org
Fragile X syndrome is caused by the absence of protein FMRP, the function of which is still
poorly understood. Previous studies have suggested that FMRP may be involved in various …

DMS footprinting of structured RNAs and RNA–protein complexes

P Tijerina, S Mohr, R Russell - Nature protocols, 2007 - nature.com
We describe a protocol in which dimethyl sulfate (DMS) modification of the base-pairing
faces of unpaired adenosine and cytidine nucleotides is used for structural analysis of RNAs …

Mod-seq: high-throughput sequencing for chemical probing of RNA structure

J Talkish, G May, Y Lin, JL Woolford, CJ McManus - Rna, 2014 - rnajournal.cshlp.org
The functions of RNA molecules are intimately linked to their ability to fold into complex
secondary and tertiary structures. Thus, understanding how these molecules fold is essential …

A novel function for fragile X mental retardation protein in translational activation

EG Bechara, MC Didiot, M Melko, L Davidovic… - PLoS …, 2009 - journals.plos.org
Fragile X syndrome, the most frequent form of inherited mental retardation, is due to the
absence of Fragile X Mental Retardation Protein (FMRP), an RNA-binding protein involved …

A single internal ribosome entry site containing a G quartet RNA structure drives fibroblast growth factor 2 gene expression at four alternative translation initiation …

S Bonnal, C Schaeffer, L Créancier, S Clamens… - Journal of Biological …, 2003 - ASBMB
The 484-nucleotide (nt) alternatively translated region (ATR) of the human fibroblast growth
factor 2 (FGF-2) mRNA contains four CUG and one AUG translation initiation codons …

Binding and cleavage of CRISPR RNA by Cas6

J Carte, NT Pfister, MM Compton, RM Terns, MP Terns - Rna, 2010 - rnajournal.cshlp.org
The CRISPR–Cas system provides many prokaryotes with acquired resistance to viruses
and other mobile genetic elements. The core components of this defense system are small …

Native top-down mass spectrometry uncovers two distinct binding motifs of a functional neomycin-sensing riboswitch aptamer

SV Heel, K Bartosik, F Juen, C Kreutz… - Journal of the …, 2023 - ACS Publications
Understanding how ligands bind to ribonucleic acids (RNA) is important for understanding
RNA recognition in biological processes and drug development. Here, we have studied …

Glyoxals as in vivo RNA structural probes of guanine base-pairing

D Mitchell, LE Ritchey, H Park, P Babitzke… - Rna, 2018 - rnajournal.cshlp.org
Elucidation of the folded structures that RNA forms in vivo is vital to understanding its
functions. Chemical reagents that modify the Watson–Crick (WC) face of unprotected …