dPCR: a technology review
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …
Passive and active droplet generation with microfluidics: a review
Precise and effective control of droplet generation is critical for applications of droplet
microfluidics ranging from materials synthesis to lab-on-a-chip systems. Methods for droplet …
microfluidics ranging from materials synthesis to lab-on-a-chip systems. Methods for droplet …
Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing
While nanoscale quantum emitters are effective tags for measuring biomolecular
interactions, their utilities for applications that demand single-unit observations are limited by …
interactions, their utilities for applications that demand single-unit observations are limited by …
Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip
Portable, low-cost, and quantitative nucleic acid detection is desirable for point-of-care
diagnostics; however, current polymerase chain reaction testing often requires time …
diagnostics; however, current polymerase chain reaction testing often requires time …
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …
practical and increasingly affordable technology. dPCR allows the precise quantification of …
Highly precise measurement of HIV DNA by droplet digital PCR
Deoxyribonucleic acid (DNA) of the human immunodeficiency virus (HIV) provides the most
sensitive measurement of residual infection in patients on effective combination antiretroviral …
sensitive measurement of residual infection in patients on effective combination antiretroviral …
Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples
Rapid antimicrobial susceptibility testing (AST) is urgently needed for informing treatment
decisions and preventing the spread of antimicrobial resistance resulting from the misuse …
decisions and preventing the spread of antimicrobial resistance resulting from the misuse …
Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications
Since the invention of polymerase chain reaction (PCR) in 1985, PCR has played a
significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and …
significant role in molecular diagnostics for genetic diseases, pathogens, oncogenes and …
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
One of the benefits of Digital PCR (dPCR) is the potential for unparalleled precision
enabling smaller fold change measurements. An example of an assessment that could …
enabling smaller fold change measurements. An example of an assessment that could …
Advances in droplet digital polymerase chain reaction on microfluidic chips
The PCR technique has been known to the general public since the pandemic outbreak of
COVID-19. This technique has progressed through three stages: from simple PCR to real …
COVID-19. This technique has progressed through three stages: from simple PCR to real …