Third-generation biorefineries as the means to produce fuels and chemicals from CO2

Z Liu, K Wang, Y Chen, T Tan, J Nielsen - Nature Catalysis, 2020 - nature.com
Concerns regarding petroleum depletion and global climate change caused by greenhouse
gas emissions have spurred interest in renewable alternatives to fossil fuels. Third …

Yeast systems biology: model organism and cell factory

J Nielsen - Biotechnology journal, 2019 - Wiley Online Library
For thousands of years, the yeast Saccharomyces cerevisiae (S. cerevisiae) has served as a
cell factory for the production of bread, beer, and wine. In more recent years, this yeast has …

Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism

J Zhang, SD Petersen, T Radivojevic, A Ramirez… - Nature …, 2020 - nature.com
Through advanced mechanistic modeling and the generation of large high-quality datasets,
machine learning is becoming an integral part of understanding and engineering living …

Controlling gene expression with deep generative design of regulatory DNA

J Zrimec, X Fu, AS Muhammad, C Skrekas… - Nature …, 2022 - nature.com
Abstract Design of de novo synthetic regulatory DNA is a promising avenue to control gene
expression in biotechnology and medicine. Using mutagenesis typically requires screening …

A consensus S. cerevisiae metabolic model Yeast8 and its ecosystem for comprehensively probing cellular metabolism

H Lu, F Li, BJ Sánchez, Z Zhu, G Li, I Domenzain… - Nature …, 2019 - nature.com
Genome-scale metabolic models (GEMs) represent extensive knowledgebases that provide
a platform for model simulations and integrative analysis of omics data. This study …

Improving the phenotype predictions of a yeast genome‐scale metabolic model by incorporating enzymatic constraints

BJ Sánchez, C Zhang, A Nilsson… - Molecular systems …, 2017 - embopress.org
Genome‐scale metabolic models (GEM s) are widely used to calculate metabolic
phenotypes. They rely on defining a set of constraints, the most common of which is that the …

Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure

J Zrimec, CS Börlin, F Buric, AS Muhammad… - Nature …, 2020 - nature.com
Understanding the genetic regulatory code governing gene expression is an important
challenge in molecular biology. However, how individual coding and non-coding regions of …

Recent advances in metabolic engineering of Saccharomyces cerevisiae: new tools and their applications

J Lian, S Mishra, H Zhao - Metabolic engineering, 2018 - Elsevier
Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Turnover number predictions for kinetically uncharacterized enzymes using machine and deep learning

A Kroll, Y Rousset, XP Hu, NA Liebrand… - Nature …, 2023 - nature.com
The turnover number k cat, a measure of enzyme efficiency, is central to understanding
cellular physiology and resource allocation. As experimental k cat estimates are unavailable …

Unification of protein abundance datasets yields a quantitative Saccharomyces cerevisiae proteome

B Ho, A Baryshnikova, GW Brown - Cell systems, 2018 - cell.com
Protein activity is the ultimate arbiter of function in most cellular pathways, and protein
concentration is fundamentally connected to protein action. While the proteome of yeast has …