Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Conspectus The sophistication of proteomic analysis has revealed that protein lysine
residues are posttranslationally modified by a variety of acyl groups. Protein lysine …

Chromatin features are widely used for genome-scale map** of enhancers. However,
discriminating active enhancers from other cis-regulatory elements, predicting enhancer …

The reversible acetylation of histone lysine residues is controlled by the action of
acetyltransferases and deacetylases (HDACs), which regulate chromatin structure and gene …

The chemical synthesis of homogeneously modified histones is a powerful approach to
quantitatively decipher how post-translational modifications (PTMs) modulate epigenetic …

Biochemical crosstalk between two or more histone modifications is often observed in
epigenetic enzyme regulation, but its functional significance in cells has been difficult to …

UM171 is a potent agonist of ex vivo human haematopoietic stem cell self-renewal. By co-
opting KBTBD4, a substrate receptor of the CUL3–RING E3 ubiquitin ligase (CRL3) …

Lysine acetyltransferase (KAT) enzymes including the p300, MYST, and GCN5 families play
major roles in modulating the structure of chromatin and regulating transcription. Because of …

Sortase A (SrtA)‐mediated ligation, a popular method for protein labeling and semi‐
synthesis, is limited by its reversibility and dependence on the LPxTG motif, where “x” is any …

The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …