Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …

Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

We describe optimized fragmentation schemes and data analysis strategies substantially
enhancing the depth and accuracy in identifying protein cross-links using non-restricted …

Chemical cross-linking in combination with mass spectrometric analysis of the created cross-
linked products is an emerging technology aimed at deriving valuable structural information …

Structural mass spectrometry (MS) is gaining increasing importance for deriving valuable
three‐dimensional structural information on proteins and protein complexes, and it …

Mitochondria exert an immense amount of cytophysiological functions, but the structural
basis of most of these processes is still poorly understood. Here we use cross-linking mass …

Mass spectrometry (MS) is increasingly being used to probe the structure and dynamics of
proteins and the complexes they form with other macromolecules. There are now several …

Highlights•Cross-linking-mass spectrometry constitutes a chemical method to produce
protein complex structural model constraints.•A wide range of sample types are applicable …

Cross-linking mass spectrometry (XL-MS) has matured into a potent tool to identify protein–
protein interactions or to uncover protein structures in living cells, tissues, or organelles. The …

Cells organize their actions partly through tightly controlled protein-protein interactions—
collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to …