Current best practices in single‐cell RNA‐seq analysis: a tutorial

MD Luecken, FJ Theis - Molecular systems biology, 2019 - embopress.org
Single‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented
resolution. The promise of this technology is attracting a growing user base for single‐cell …

The Perseus computational platform for comprehensive analysis of (prote) omics data

S Tyanova, T Temu, P Sinitcyn, A Carlson, MY Hein… - Nature …, 2016 - nature.com
A main bottleneck in proteomics is the downstream biological analysis of highly multivariate
quantitative protein abundance data generated using mass-spectrometry-based analysis …

Welcome to the Tidyverse

H Wickham, M Averick, J Bryan, W Chang… - Journal of open source …, 2019 - joss.theoj.org
At a high level, the tidyverse is a language for solving data science challenges with R code.
Its primary goal is to facilitate a conversation between a human and a computer about data …

ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R

E Paradis, K Schliep - Bioinformatics, 2019 - academic.oup.com
After more than fifteen years of existence, the R package ape has continuously grown its
contents, and has been used by a growing community of users. The release of version 5.0 …

Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

E Bolyen, JR Rideout, MR Dillon, NA Bokulich… - Nature …, 2019 - nature.com
To the Editor—Rapid advances in DNA-sequencing and bioinformatics technologies in the
past two decades have substantially improved understanding of the microbial world. This …

SCANPY: large-scale single-cell gene expression data analysis

FA Wolf, P Angerer, FJ Theis - Genome biology, 2018 - Springer
Scanpy is a scalable toolkit for analyzing single-cell gene expression data. It includes
methods for preprocessing, visualization, clustering, pseudotime and trajectory inference …

[HTML][HTML] The R package Rsubread is easier, faster, cheaper and better for alignment and quantification of RNA sequencing reads

Y Liao, GK Smyth, W Shi - Nucleic acids research, 2019 - academic.oup.com
Abstract We present Rsubread, a Bioconductor software package that provides high-
performance alignment and read counting functions for RNA-seq reads. Rsubread is based …

Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown

M Pertea, D Kim, GM Pertea, JT Leek, SL Salzberg - Nature protocols, 2016 - nature.com
High-throughput sequencing of mRNA (RNA-seq) has become the standard method for
measuring and comparing the levels of gene expression in a wide variety of species and …

[HTML][HTML] Spatial transcriptomics and in situ sequencing to study Alzheimer's disease

WT Chen, A Lu, K Craessaerts, B Pavie, CS Frigerio… - Cell, 2020 - cell.com
Although complex inflammatory-like alterations are observed around the amyloid plaques of
Alzheimer's disease (AD), little is known about the molecular changes and cellular …

TCGAbiolinks: an R/Bioconductor package for integrative analysis of TCGA data

A Colaprico, TC Silva, C Olsen, L Garofano… - Nucleic acids …, 2016 - academic.oup.com
Abstract The Cancer Genome Atlas (TCGA) research network has made public a large
collection of clinical and molecular phenotypes of more than 10 000 tumor patients across …