Mitochondria are cellular organelles responsible for generation of chemical energy in the
process called oxidative phosphorylation. They originate from a bacterial ancestor and …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Cryo-electron microscopy (cryo-EM) single-particle analysis has proven powerful in
determining the structures of rigid macromolecules. However, many imaged protein …

Single particle cryo-EM excels in determining static structures of protein molecules, but
existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We …

Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of
protein function. Cryogenic electron microscopy (cryo-EM) provides direct visualization of …

Single-particle cryogenic electron microscopy (cryo-EM) has emerged as a powerful
technique to visualize the structural landscape sampled by a protein complex. However …

We present a strategy for tackling preferred specimen orientation in single-particle cryogenic
electron microscopy by employing tilts during data collection. We also describe a tool to …

Assembly of ribosomes in bacteria is highly efficient, taking~ 2-3 min, but this makes the
abundance of assembly intermediates very low, which is a challenge for mechanistic …

Nitrite (NO2–) accumulation caused by nitrite-oxidizing bacteria (NOB) inhibition in
nitrification is a double-edged sword, ie, a disaster in aquatic environments but a hope for …

Ribosome biogenesis is a fundamental multi-step cellular process in all domains of life that
involves the production, processing, folding, and modification of ribosomal RNAs (rRNAs) …