Isotachophoresis: theory and microfluidic applications

A Ramachandran, JG Santiago - Chemical Reviews, 2022 - ACS Publications
Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample
preconcentration, separation, purification, and mixing, and to control and accelerate …

Purification of nucleic acids using isotachophoresis

A Rogacs, LA Marshall, JG Santiago - Journal of Chromatography A, 2014 - Elsevier
Reviewed are methods of nucleic acid (NA) extraction and sample preparation using an
electrophoretic purification and focusing method called isotachophoresis (ITP). ITP requires …

Two orders of magnitude improvement in detection limit of lateral flow assays using isotachophoresis

BY Moghadam, KT Connelly, JD Posner - Analytical chemistry, 2015 - ACS Publications
Lateral flow immunoassays (LFA) are one of the most prevalent point-of-care (POC)
diagnostics due to their simplicity, low cost, and robust operation. A common criticism of LFA …

Isotachophoretic preconcenetration on paper-based microfluidic devices

BY Moghadam, KT Connelly, JD Posner - Analytical chemistry, 2014 - ACS Publications
Paper substrates have been widely used to construct point-of-care lateral flow immunoassay
(LFIA) diagnostic devices. Paper based microfluidic devices are robust and relatively simple …

Simulation of chaotic electrokinetic transport: Performance of commercial software versus custom-built direct numerical simulation codes

E Karatay, CL Druzgalski, A Mani - Journal of colloid and interface science, 2015 - Elsevier
Many microfluidic and electrochemical applications involve chaotic transport phenomena
that arise due to instabilities stemming from coupling of hydrodynamics with ion transport …

Rapid hybridization of nucleic acids using isotachophoresis

M Bercovici, CM Han, JC Liao… - Proceedings of the …, 2012 - National Acad Sciences
We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization
reactions in free solution. We present a new physical model, validation experiments, and …

HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification

AT Bender, BP Sullivan, JY Zhang, DC Juergens… - Analyst, 2021 - pubs.rsc.org
The number of people living with HIV continues to increase with the current total near 38
million, of which about 26 million are receiving antiretroviral therapy (ART). These treatment …

Coupling isotachophoresis and capillary electrophoresis: a review and comparison of methods

SS Bahga, JG Santiago - Analyst, 2013 - pubs.rsc.org
We present a comprehensive review and comparison of the methodologies for increasing
sensitivity and resolution of capillary electrophoresis (CE) using online transient …

Semiquantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification

AT Bender, MD Borysiak, AM Levenson, L Lillis… - Analytical …, 2018 - ACS Publications
Nucleic acid amplification tests (NAATs) provide high diagnostic accuracy for infectious
diseases and quantitative results for monitoring viral infections. The majority of NAATs …

Electrophoretic mobility measurements of fluorescent dyes using on‐chip capillary electrophoresis

D Milanova, RD Chambers, SS Bahga… - …, 2011 - Wiley Online Library
We present an experimental study of the effect of pH, ionic strength, and concentrations of
the electroosmotic flow (EOF)‐suppressing polymer polyvinylpyrrolidone (PVP) on the …