Isotachophoresis: theory and microfluidic applications
A Ramachandran, JG Santiago - Chemical Reviews, 2022 - ACS Publications
Isotachophoresis (ITP) is a versatile electrophoretic technique that can be used for sample
preconcentration, separation, purification, and mixing, and to control and accelerate …
preconcentration, separation, purification, and mixing, and to control and accelerate …
Purification of nucleic acids using isotachophoresis
Reviewed are methods of nucleic acid (NA) extraction and sample preparation using an
electrophoretic purification and focusing method called isotachophoresis (ITP). ITP requires …
electrophoretic purification and focusing method called isotachophoresis (ITP). ITP requires …
Two orders of magnitude improvement in detection limit of lateral flow assays using isotachophoresis
BY Moghadam, KT Connelly, JD Posner - Analytical chemistry, 2015 - ACS Publications
Lateral flow immunoassays (LFA) are one of the most prevalent point-of-care (POC)
diagnostics due to their simplicity, low cost, and robust operation. A common criticism of LFA …
diagnostics due to their simplicity, low cost, and robust operation. A common criticism of LFA …
Isotachophoretic preconcenetration on paper-based microfluidic devices
BY Moghadam, KT Connelly, JD Posner - Analytical chemistry, 2014 - ACS Publications
Paper substrates have been widely used to construct point-of-care lateral flow immunoassay
(LFIA) diagnostic devices. Paper based microfluidic devices are robust and relatively simple …
(LFIA) diagnostic devices. Paper based microfluidic devices are robust and relatively simple …
Simulation of chaotic electrokinetic transport: Performance of commercial software versus custom-built direct numerical simulation codes
Many microfluidic and electrochemical applications involve chaotic transport phenomena
that arise due to instabilities stemming from coupling of hydrodynamics with ion transport …
that arise due to instabilities stemming from coupling of hydrodynamics with ion transport …
Rapid hybridization of nucleic acids using isotachophoresis
We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization
reactions in free solution. We present a new physical model, validation experiments, and …
reactions in free solution. We present a new physical model, validation experiments, and …
HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification
The number of people living with HIV continues to increase with the current total near 38
million, of which about 26 million are receiving antiretroviral therapy (ART). These treatment …
million, of which about 26 million are receiving antiretroviral therapy (ART). These treatment …
Coupling isotachophoresis and capillary electrophoresis: a review and comparison of methods
SS Bahga, JG Santiago - Analyst, 2013 - pubs.rsc.org
We present a comprehensive review and comparison of the methodologies for increasing
sensitivity and resolution of capillary electrophoresis (CE) using online transient …
sensitivity and resolution of capillary electrophoresis (CE) using online transient …
Semiquantitative nucleic acid test with simultaneous isotachophoretic extraction and amplification
AT Bender, MD Borysiak, AM Levenson, L Lillis… - Analytical …, 2018 - ACS Publications
Nucleic acid amplification tests (NAATs) provide high diagnostic accuracy for infectious
diseases and quantitative results for monitoring viral infections. The majority of NAATs …
diseases and quantitative results for monitoring viral infections. The majority of NAATs …
Electrophoretic mobility measurements of fluorescent dyes using on‐chip capillary electrophoresis
D Milanova, RD Chambers, SS Bahga… - …, 2011 - Wiley Online Library
We present an experimental study of the effect of pH, ionic strength, and concentrations of
the electroosmotic flow (EOF)‐suppressing polymer polyvinylpyrrolidone (PVP) on the …
the electroosmotic flow (EOF)‐suppressing polymer polyvinylpyrrolidone (PVP) on the …