Engineered polyethylene terephthalate hydrolases: perspectives and limits

F Kawai, R Iizuka, T Kawabata - Applied Microbiology and Biotechnology, 2024 - Springer
Polyethylene terephthalate (PET) is a major component of plastic waste. Enzymatic PET
hydrolysis is the most ecofriendly recycling technology. The biorecycling of PET waste …

Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation

G Liu, H Yuan, Y Chen, L Mao, C Yang, R Zhang… - International Journal of …, 2024 - Elsevier
The proliferation of nano-plastic particles (NPs) poses severe environmental hazards,
urgently requiring effective biodegradation methods. Herein, a novel method was developed …

Synergistic functional activity of a landfill microbial consortium in a microplastic-enriched environment

A Satta, G Ghiotto, D Santinello, G Giangeri… - Science of the Total …, 2024 - Elsevier
Plastic pollution of the soil is a global issue of increasing concern, with far-reaching impact
on the environment and human health. To fully understand the medium-and long-term …

[HTML][HTML] Protein Engineering for Industrial Biocatalysis: Principles, Approaches, and Lessons from Engineered PETases

K Grigorakis, C Ferousi, E Topakas - Catalysts, 2025 - mdpi.com
Protein engineering has emerged as a transformative field in industrial biotechnology,
enabling the optimization of enzymes to meet stringent industrial demands for stability …

Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli …

S Fritzsche, H Hübner, M Oldiges, K Castiglione - Microbial Cell Factories, 2024 - Springer
Background With a growing global population, the generation of plastic waste and the
depletion of fossil resources are major concerns that need to be addressed by develo** …

Unusual depolymerization mechanism of Poly (ethylene terephthalate) by hydrolase 202

N Wang, Y Li, M Zheng, W Dong, Q Zhang, W Wang - Chemosphere, 2025 - Elsevier
Polyethylene terephthalate (PET) waste significantly contributes to the global plastic crisis,
but enzymatic conversion has become an efficient and environmentally friendly strategy to …

Adaptive laboratory evolution and genetic engineering improved terephthalate utilization in Pseudomonas putida KT2440

AZ Werner, YSC Avina, J Johnsen, F Bratti, HM Alt… - Metabolic …, 2024 - Elsevier
Abstract Poly (ethylene terephthalate)(PET) is one of the most ubiquitous plastics and can
be depolymerized through biological and chemo-catalytic routes to its constituent …

Unlocking a Key Residue in a Lipase for Efficient Polyethylene Terephthalate (PET) Hydrolysis and Influencing Depolymerization Product Profiles

P Vidal, A Robles‐Martín, L Fernandez‐Lopez… - …, 2024 - Wiley Online Library
Polyethylene terephthalate (PET) pollution is a global challenge. Advancing the
bioprospecting of PET‐degrading enzymes through metagenomics and using computational …

Fusion of Hydrophobic Anchor Peptides Promotes the Hydrolytic Activity of PETase but not the Extent of PET Depolymerization

Y Wang, E Akram, Y Ding, C He, Y Zhang - ChemCatChem - Wiley Online Library
Enzymatic recycling of polyethylene terephthalate (PET) has attracted significant attention in
recent years. While the fusion of anchor peptides to PET hydrolases is believed to enhance …