Noncanonical amino acids in biocatalysis

Z Birch-Price, FJ Hardy, TM Lister, AR Kohn… - Chemical …, 2024 - ACS Publications
In recent years, powerful genetic code reprogramming methods have emerged that allow
new functional components to be embedded into proteins as noncanonical amino acid …

Enzyme entrapment, biocatalyst immobilization without covalent attachment

HT Imam, PC Marr, AC Marr - Green Chemistry, 2021 - pubs.rsc.org
In entrapment an active species, which is often a catalyst, is trapped within a material by a
solid or gel forming event; thus, it becomes dispersed within the solid or semi-solid matrix …

The quantum chemical cluster approach in biocatalysis

X Sheng, F Himo - Accounts of Chemical Research, 2023 - ACS Publications
Conspectus The quantum chemical cluster approach has been used for modeling enzyme
active sites and reaction mechanisms for more than two decades. In this methodology, a …

Learning epistasis and residue coevolution patterns: Current trends and future perspectives for advancing enzyme engineering

M Wittmund, F Cadet, MD Davari - ACS catalysis, 2022 - ACS Publications
Engineering proteins and enzymes with the desired functionality has broad applications in
molecular biology, biotechnology, biomedical sciences, health, and medicine. The vastness …

Hot spots-making directed evolution easier

H Yu, S Ma, Y Li, PA Dalby - Biotechnology Advances, 2022 - Elsevier
Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

NMR-guided directed evolution

S Bhattacharya, EG Margheritis, K Takahashi… - Nature, 2022 - nature.com
Directed evolution is a powerful tool for improving existing properties and imparting
completely new functionalities to proteins,,–. Nonetheless, its potential in even small …

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET

RP Magalhães, JM Cunha, SF Sousa - International Journal of Molecular …, 2021 - mdpi.com
Plastics are highly durable and widely used materials. Current methodologies of plastic
degradation, elimination, and recycling are flawed. In recent years, biodegradation (the …

[HTML][HTML] Green catalysis for chemical transformation: The need for the sustainable development

A Kate, LK Sahu, J Pandey, M Mishra… - Current Research in …, 2022 - Elsevier
The worldwide concern for the sustainable future along with the advancement in the green
products with biotechnological routes has enhanced the utilization of bio-catalysis also …

Importance of enzyme specificity and stability for the application of proteases in greener industrial processing-a review

P Sujitha, C Shanthi - Journal of Cleaner Production, 2023 - Elsevier
Industries are in great need for process-apt proteases due to environmental concerns. But
the major bottlenecks in the use of enzymes in industrial processes are their lack of …

Mechanism and Application of Surface-Charged Ferrite Nanozyme-Based Biosensor toward Colorimetric Detection of l-Cysteine

H Wu, J Liu, Z Chen, P Lin, W Ou, Z Wang, W **ao… - Langmuir, 2022 - ACS Publications
Peroxidase-like nanozymes with robust catalytic capacity and detection specificity have
been proposed as substitutes to natural peroxidases in biochemical sensing. However, the …