Integrating CRISPR/Cas within isothermal amplification for point-of-Care Assay of nucleic acid

L Zhang, H Jiang, Z Zhu, J Liu, B Li - Talanta, 2022 - Elsevier
Nucleic acid detection technology is now widely used in scientific research and clinical
testing, such as infectious and genetic diseases screening, molecular diagnosis of tumors …

CRISPR-based biosensors for pathogenic biosafety

H Yang, R Ledesma-Amaro, H Gao, Y Ren… - Biosensors and …, 2023 - Elsevier
Pathogenic biosafety is a worldwide concern. Tools for analyzing pathogenic biosafety, that
are precise, rapid and field-deployable, are highly demanded. Recently developed …

Uniform stain pattern of robust MOF-mediated probe for flexible paper-based colorimetric sensing toward environmental pesticide exposure

X Zhang, Z Wang, X Huang, Q Huang, Y Wen… - Chemical Engineering …, 2023 - Elsevier
Excessive residual pesticides have raised overall environmental damage and health hazard
issues, thus the development of facile and sensitive means for pesticide monitoring in …

Pyrococcus furiosus Argonaute Combined with Recombinase Polymerase Amplification for Rapid and Sensitive Detection of Enterocytozoon hepatopenaei

L Yang, B Guo, Y Wang, C Zhao, X Zhang… - Journal of Agricultural …, 2022 - ACS Publications
Enterocytozoon hepatopenaei (EHP) is one of the most serious pathogens in shrimp
farming. This study combines recombinase polymerase amplification (RPA) with the …

CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases

Y He, Q Hu, S San, T Kasputis, MGD Splinter… - TrAC Trends in …, 2023 - Elsevier
Infectious diseases (such as sepsis, influenza, and malaria), caused by various pathogenic
bacteria and viruses, are widespread across the world. Early and rapid detection of disease …

One-pot molecular diagnosis of acute hepatopancreatic necrosis disease by recombinase polymerase amplification and CRISPR/Cas12a with specially designed …

P Wang, B Guo, X Zhang, Y Wang, G Yang… - Journal of Agricultural …, 2023 - ACS Publications
Acute hepatopancreatic necrosis disease (AHPND) is one of the most devastating diseases
in aquaculture, causing significant economic losses in seafood supplies worldwide. Early …

CRISPR/Cas-based diagnostics in agricultural applications

T Tanny, M Sallam, N Soda, NT Nguyen… - Journal of Agricultural …, 2023 - ACS Publications
Pests and disease-causing pathogens frequently impede agricultural production. An early
and efficient diagnostic tool is crucial for effective disease management. Clustered regularly …

Recent Progress in Molecular Diagnostics: The Synergy of Rolling Circle Amplification and CRISPR/Cas Systems (2018-2024)–A Concise Review

Z Zhu, L Yang - TrAC Trends in Analytical Chemistry, 2024 - Elsevier
Abstract The fusion of Rolling Circle Amplification (RCA) and the Clustered Regularly
Interspaced Short Palindromic Repeats–CRISPR-associated proteins (CRISPR-Cas) stands …

Csm6-DNAzyme tandem assay for one-pot and sensitive analysis of lead pollution and bioaccumulation in mice

H Yang, F Li, T Xue, MR Khan, X **a… - Analytical …, 2022 - ACS Publications
Lead contamination in the environment tends to enter the food chain and further into the
human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme …

Rapid in situ RNA imaging based on Cas12a thrusting strand displacement reaction

X Cheng, X Li, Y Kang, D Zhang, Q Yu… - Nucleic Acids …, 2023 - academic.oup.com
RNA In situ imaging through DNA self-assembly is advantaged in illustrating its structures
and functions with high-resolution, while the limited reaction efficiency and time-consuming …