In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Intrinsically disordered proteins (IDPs) are unable to adopt a unique 3D structure under
physiological conditions and thus exist as highly dynamic conformational ensembles. IDPs …

Site-directed spin-labeling (SDSL)─ in combination with double electron–electron
resonance (DEER) spectroscopy─ has emerged as a powerful technique for determining …

Abstract Site-Directed Spin Labelling (SDSL) technique is based on the attachment of a
paramagnetic label onto a specific position of a protein (or other bio-molecules) and the …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

The electron–electron double resonance (DEER) method, which provides distance
distributions between two spin labels, attached site specifically to biomolecules (proteins …

The understanding of biomolecular function is coupled to knowledge about the structure and
dynamics of these biomolecules, preferably acquired under native conditions. In this regard …

Studies of protein structure and dynamics are usually carried out in dilute buffer solutions,
conditions that differ significantly from the crowded environment in the cell. The double …

The study of how the intracellular medium influences protein structural dynamics and protein–
protein interactions is a captivating area of research for scientists aiming to comprehend …

Exploring the structure and dynamics of biomolecules in the context of their intracellular
environment has become the ultimate challenge for structural biology. As the cellular …