The polymerase chain reaction (PCR) is increasingly used as the standard method for
detection and characterization of microorganisms and genetic markers in a variety of sample …

Factors that inhibit the amplification of nucleic acids by PCR are present with target DNAs
from many sources. The inhibitors generally act at one or more of three essential points in …

Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform
assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is …

In a recent study, immunoglobulin G in human plasma was identified as a major inhibitor of
diagnostic PCR (W. Abu Al-Soud, LJ Jönsson, and P. Rådström. J. Clin. Microbiol. 38 …

The benefits of adding bovine serum albumin (BSA) or T4 gene 32 protein (gp32) to PCR
were evaluated with reaction mixtures containing substances that inhibit amplification …

Three sets of primers were designed for PCR detection and differentiation of Campylobacter
jejuni and Campylobacter coli. The first PCR assay was designed to coidentify C. jejuni and …

Magnetic separation is an emerging technology using magnetism, sometimes in
combination with conventional separation or identification methods, to purify cells, cell …

The principles of magnetic separation aided by antibodies or other specific binding
molecules have been used for isolation of specific viable whole organisms, antigens, or …

The early diagnosis of diseases, eg, Parkinson's and Alzheimer's disease, diabetes, and
various types of cancer, and monitoring the response of patients to the therapy plays a …

In order to make a rapid and definite diagnosis of Salmonella enteritis in children, an
enrichment broth culture-multiplex PCR combination assay was devised to identify …