Chemical approaches to cryopreservation

KA Murray, MI Gibson - Nature Reviews Chemistry, 2022 - nature.com
Cryopreservation of cells and biologics underpins all biomedical research from routine
sample storage to emerging cell-based therapies, as well as ensuring cell banks provide …

Digital microfluidics

K Choi, AHC Ng, R Fobel… - Annual review of …, 2012 - annualreviews.org
Digital microfluidics (DMF) is an emerging liquid-handling technology that enables
individual control over droplets on an open array of electrodes. These picoliter-to microliter …

Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity?

M Awan, I Buriak, R Fleck, B Fuller, A Goltsev… - Regenerative …, 2020 - Taylor & Francis
Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since
the early history of cryopreservation. It has been used for decades in many thousands of cell …

Microfluidics for cryopreservation

G Zhao, J Fu - Biotechnology advances, 2017 - Elsevier
Cryopreservation has utility in clinical and scientific research but implementation is highly
complex and includes labor-intensive cell-specific protocols for the addition/removal of …

Advances in microfluidics‐based assisted reproductive technology: From sperm sorter to reproductive system‐on‐a‐chip

N Kashaninejad, MJA Shiddiky… - Advanced …, 2018 - Wiley Online Library
The fields of assisted reproductive technology (ART) and in vitro fertilization (IVF) have
progressed rapidly, yet still need further improvements. Microfluidic technology can …

A digital microfluidic system with 3D microstructures for single-cell culture

J Zhai, H Li, AHH Wong, C Dong, S Yi, Y Jia… - Microsystems & …, 2020 - nature.com
Despite the precise controllability of droplet samples in digital microfluidic (DMF) systems,
their capability in isolating single cells for long-time culture is still limited: typically, only a few …

An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays

C Dixon, AHC Ng, R Fobel, MB Miltenburg… - Lab on a Chip, 2016 - pubs.rsc.org
The diagnosis of infectious disease is typically carried out at the point-of-care (POC) using
the lateral flow assay (LFA). While cost-effective and portable, LFAs often lack the clinical …

Antifouling properties of pluronic and tetronic surfactants in digital microfluidics

M Ho, A Au, R Flick, TV Vuong… - … Applied Materials & …, 2023 - ACS Publications
Fouling at liquid–solid interfaces is a pernicious problem for a wide range of applications,
including those that are implemented by digital microfluidics (DMF). There are several …

An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR

A Rival, D Jary, C Delattre, Y Fouillet, G Castellan… - Lab on a Chip, 2014 - pubs.rsc.org
Single cell analysis circumvents the need to average data from large populations by
observing each cell individually, thus enabling the analysis of cell-to-cell variability. The …

Microfluidic-based droplets for advanced regenerative medicine: Current challenges and future trends

H Nazari, A Heirani-Tabasi, S Ghorbani, H Eyni… - Biosensors, 2021 - mdpi.com
Microfluidics is a promising approach for the facile and large-scale fabrication of
monodispersed droplets for various applications in biomedicine. This technology has …