Cellular DNA is continuously transcribed into RNA by multisubunit RNA polymerases
(RNAPs). The continuity of transcription can be disrupted by DNA lesions that arise from the …

Crosslinking mass spectrometry captures protein structures in solution. The crosslinks reveal
spatial proximities as distance restraints, but do not easily reveal which of these restraints …

Fidaxomicin (Fdx) is widely used to treat Clostridioides difficile (Cdiff) infections, but the
molecular basis of its narrow-spectrum activity in the human gut microbiome remains …

Efficient and accurate termination is required for gene transcription in all living organisms,.
Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription …

While photo-cross-linking (PXL) with alkyl diazirines can provide stringent distance restraints
and offer insights into protein structures, unambiguous identification of cross-linked residues …

RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA
sequences signal RNA polymerase (RNAP) to pause transcription and are required for …

Spx is a global transcriptional regulator in Gram-positive bacteria and has been inferred to
efficiently activate transcription upon oxidative stress by engaging RNA polymerase (RNAP) …

In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks
for the DNA replication machinery that must be removed in order to prevent damaging …

RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors
for recovery from stalled states and adaptation to environmental changes. Here, we …

Bacterial RNAP needs to form holoenzyme with σ factors to initiate transcription. While
Staphylococcus aureus σA controls housekee** functions, S. aureus σB regulates …