This paper sheds light on the meaning of hydrogen/deuterium exchange-mass spectrometry
(HDX-MS) data. HDX-MS data provide not structural information but dynamic information on …

Biotherapeutics and their biosimilar versions have been flourishing in the biopharmaceutical
market for several years. Structural and functional characterization is needed to achieve …

Fc γ receptors (FcγRs) are one of the structures that can initiate effector function for
monoclonal antibodies. FcγRIa has the highest affinity toward IgG1-type monoclonal …

Chromatographic separations at subzero temperature significantly improve the precision of
back-exchange-corrected hydrogen–deuterium exchange mass spectrometry (HDX-MS) …

Hydrogen–deuterium exchange coupled with mass spectrometry (HDX–MS) is widely used
for monoclonal antibody (mAb) epitope map**, which aids in the development of …

Although interactions of small molecular drugs with serum proteins have been widely
studied from pharmacokinetic and pharmacodynamic perspectives, there have been few …

FcγRI plays a crucial role in the effector function of IgG antibodies, interacting with the lower
hinge region of IgG1 with nanomolar affinity. Binding occurs specifically in domain 2 (D2) of …

Biopharmaceuticals such as monoclonal antibodies are required to be rigorously
characterized using a wide range of analytical methods. Various material properties must be …

Vascular endothelial growth factor (VEGF) is a critical angiogenesis biomarker associated
with various pathological conditions, including cancer. This study leverages pre-biotinylated …

Capillary vibrating sharp-edge spray ionization (cVSSI) combined with hydrogen/deuterium
exchange-mass spectrometry (HDX-MS) has been utilized to characterize different solution …