Fluorescence lifetime imaging microscopy

B Torrado, B Pannunzio, L Malacrida… - Nature Reviews …, 2024 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

The damaging effects of long UVA (UVA1) rays: a major challenge to preserve skin health and integrity

F Bernerd, T Passeron, I Castiel… - International journal of …, 2022 - mdpi.com
Within solar ultraviolet (UV) light, the longest UVA1 wavelengths, with significant and
relatively constant levels all year round and large penetration properties, produce effects in …

Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis

E Sánchez-Ramírez, TPL Ung… - Journal of Cell …, 2022 - rupress.org
Adipocytes are the main cell type in adipose tissue, which is a critical regulator of
metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from …

In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses

AM Pena, E Decencière, S Brizion, P Sextius… - Scientific Reports, 2022 - nature.com
Characterizing melanins in situ and determining their 3D z-epidermal distribution is
paramount for understanding physiological/pathological processes of melanin neosynthesis …

Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for …

MJ García, A Kamaid, L Malacrida - Biophysical Reviews, 2023 - Springer
Over the past decade, the utilization of advanced fluorescence microscopy technologies has
presented numerous opportunities to study or re-investigate autofluorescent molecules and …

Multiphoton FLIM analyses of native and UVA-modified synthetic melanins

AM Pena, S Ito, T Bornschlögl, S Brizion… - International Journal of …, 2023 - mdpi.com
To better understand the impact of solar light exposure on human skin, the chemical
characterization of native melanins and their structural photo-modifications is of central …

Label-free spatially maintained measurements of metabolic phenotypes in cells

L Hu, N Wang, JD Bryant, L Liu, L **e… - … in Bioengineering and …, 2023 - frontiersin.org
Metabolic reprogramming at a cellular level contributes to many diseases including cancer,
yet few assays are capable of measuring metabolic pathway usage by individual cells within …

[HTML][HTML] Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway

M Mei, R Cai, Q Yu, R Tian, W Zhu, J Song… - Journal of Functional …, 2023 - Elsevier
Background Salidroside (SAL) is the main component of Renus Rhodiola, Studies have
shown that it has anti-inflammation, reduces oxidative stress and anti-aging effect. However …

The effect of carnosine on UVA-induced changes in intracellular signaling of human skin fibroblast spheroids

G Aiello, F Rescigno, M Meloni, B Zoanni, G Aldini… - Antioxidants, 2023 - mdpi.com
Dermis fibroblasts are very sensitive to penetrating UVA radiation and induce photo-
damage. To protect skin cells against this environmental damage, there is an urgent need …

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

G Hilzenrat, ET Gill, SL McArthur - Journal of Biophotonics, 2022 - Wiley Online Library
The past decade has seen an increasing demand for more complex, reproducible and
physiologically relevant tissue cultures that can mimic the structural and biological features …