Click chemistry is a conceptual strategy to rapidly synthesize and discover functional
molecules. Sulfur fluoride exchange (SuFEx) is a click reaction that has revolutionized …

Interactions among biomacromolecules, predominantly noncovalent, underpin biological
processes. However, recent advancements in biospecific chemistry have enabled the …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Protein–RNA interactions regulate RNA fate and function, and defects can lead to various
disorders. Such interactions have mainly been studied by nucleoside-based UV crosslinking …

Sulfur (VI)‐fluoride exchange linkage as a next generation of click chemistry was introduced
by Sharpless and coworkers in 2014. Distinguished from CuAAC, the SuFEx reaction …

Aptamer-based detection and therapy have made substantial progress with cost control and
easy modification. However, the conformation lability of an aptamer typically causes the …

Methods rooted in chemical biology have contributed significantly to studies of integral
membrane proteins. One recent key approach has been the application of genetic code …

Sulfonyl fluorides display unique reactivity in organic synthesis and have wide applications
in drug discovery, chemical biology, and materials science. Against the backdrop of …

Sulfur fluoride exchange (SuFEx) and phosphorus fluoride exchange (PFEx) click
chemistries are advancing research across multiple disciplines. By genetically incorporating …

Noncanonical amino acids (ncAAs) for photo‐and chemical crosslinking are powerful
biochemical tools for studying and manipulating interactions between proteins both in vitro …