Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Active droplet sorting in microfluidics: a review

HD **, H Zheng, W Guo, AM Gañán-Calvo, Y Ai… - Lab on a Chip, 2017 - pubs.rsc.org
The ability to manipulate and sort droplets is a fundamental issue in droplet-based
microfluidics. Various lab-on-a-chip applications can only be realized if droplets are …

Recent advances in the use of microfluidic technologies for single cell analysis

TW Murphy, Q Zhang, LB Naler, S Ma, C Lu - Analyst, 2018 - pubs.rsc.org
The inherent heterogeneity in cell populations has become of great interest and importance
as analytical techniques have improved over the past decades. With the advent of …

Droplet morphometry and velocimetry (DMV): a video processing software for time-resolved, label-free tracking of droplet parameters

AS Basu - Lab on a Chip, 2013 - pubs.rsc.org
Emerging assays in droplet microfluidics require the measurement of parameters such as
drop size, velocity, trajectory, shape deformation, fluorescence intensity, and others. While …

Fluorescence activated cell sorting via a focused traveling surface acoustic beam

Z Ma, Y Zhou, DJ Collins, Y Ai - Lab on a Chip, 2017 - pubs.rsc.org
Fluorescence activated cell sorting (FACS) has become an essential technique widely
exploited in biological studies and clinical applications. However, current FACS systems are …

Image-based single cell sorting automation in droplet microfluidics

M Sesen, G Whyte - Scientific reports, 2020 - nature.com
The recent boom in single-cell omics has brought researchers one step closer to
understanding the biological mechanisms associated with cell heterogeneity. Rare cells that …

Micro total analysis systems: fundamental advances and biological applications

CT Culbertson, TG Mickleburgh… - Analytical …, 2014 - ACS Publications
It has been more than 20 years since the first micro total analysis systems (μTAS) papers
were published. Initial reports of these devices, which are also commonly referred to as …

Droplet microfluidics in (bio) chemical analysis

EY Basova, F Foret - Analyst, 2015 - pubs.rsc.org
Droplet microfluidics may soon change the paradigm of performing chemical analyses and
related instrumentation. It can improve not only the analysis scale, possibility for sensitivity …

Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter

TH Wu, Y Chen, SY Park, J Hong, T Teslaa, JF Zhong… - Lab on a Chip, 2012 - pubs.rsc.org
We report a high speed and high purity pulsed laser triggered fluorescence activated cell
sorter (PLACS) with a sorting throughput up to 20 000 mammalian cells s− 1 with 37 …

Droplet-based microfluidics: enabling impact on drug discovery

OJ Dressler, RM Maceiczyk… - Journal of …, 2014 - journals.sagepub.com
Over the past two decades, the application of microengineered systems in the chemical and
biological sciences has transformed the way in which high-throughput experimentation is …