Microfluidic epigenomic map** technologies for precision medicine

C Deng, LB Naler, C Lu - Lab on a Chip, 2019‏ - pubs.rsc.org
Epigenomic map** of tissue samples generates critical insights into genome-wide
regulations of gene activities and expressions during normal development and disease …

Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast

M Wang, J Li, Y Wang, H Fu, H Qiu, Y Li, M Li, Y Lu… - MBio, 2023‏ - journals.asm.org
Linker histone H1 plays a crucial role in various biological processes, including nucleosome
stabilization, high-order chromatin structure organization, gene expression, and epigenetic …

Archaeal chromatin proteins Cren7 and Sul7d compact DNA by bending and bridging

Z Zhang, Z Zhan, B Wang, Y Chen, X Chen, C Wan… - MBio, 2020‏ - journals.asm.org
Archaeal chromatin proteins Cren7 and Sul7d from Sulfolobus are DNA benders. To better
understand their architectural roles in chromosomal DNA organization, we analyzed DNA …

Utilizing biotinylated proteins expressed in yeast to visualize DNA–protein interactions at the single-molecule level

H Xue, Y Bei, Z Zhan, X Chen, X Xu, YV Fu - Frontiers in microbiology, 2017‏ - frontiersin.org
Much of our knowledge in conventional biochemistry has derived from bulk assays.
However, many stochastic processes and transient intermediates are hidden when …

Kinetic analysis of DNA compaction by mycobacterial integration host factor at the single-molecule level

Y Chen, Z Zhan, H Zhang, L Bi, XE Zhang, YV Fu - Tuberculosis, 2019‏ - Elsevier
Nucleoid-associated proteins (NAPs) play an important role on chromosome condensation
and organization. Mycobacterial integration host factor (mIHF) is one of the few …

Visualizing the interaction between the Qdot-labeled protein and site-specifically modified Λ DNA at the single molecule level

H Xue, Z Zhan, K Zhang, YV Fu - Journal of Visualized …, 2018‏ - pmc.ncbi.nlm.nih.gov
The fluorescence microscopy has made great contributions in dissecting the mechanisms of
complex biological processes at the single molecule level. In single molecule assays for …

Yeast nucleoplasmic extracts and an application to visualize chromatin assembly on single molecules of DNA

Y Wang, YV Fu - Yeast Protocols, 2021‏ - Springer
In eukaryotic cells, the genomic DNA is packaged into chromatin, the basic unit of which is
the nucleosome. Studying the mechanism of chromatin formation under physiological …

Nonequilibrium and nonlinear kinetics as key determinants for bistability in fission yeast G2-M transition

D Zhao, T Wang, J Zhao, D Li, Z Lin, Z Chen… - arxiv preprint arxiv …, 2016‏ - arxiv.org
A living cell is an open, nonequilibrium biochemical system where ATP hydrolysis serves as
the energy source for a wide range of intracellular processes, possibly including the …