[HTML][HTML] Exploring cellular biochemistry with nanobodies

RW Cheloha, TJ Harmand, C Wijne… - Journal of Biological …, 2020 - Elsevier
Reagents that bind tightly and specifically to biomolecules of interest remain essential in the
exploration of biology and in their ultimate application to medicine. Besides ligands for …

Nanobody-based probes for subcellular protein identification and visualization

MA De Beer, BNG Giepmans - Frontiers in cellular neuroscience, 2020 - frontiersin.org
Understanding how building blocks of life contribute to physiology is greatly aided by protein
identification and cellular localization. The two main labeling approaches developed over …

An improved yeast surface display platform for the screening of nanobody immune libraries

T Uchański, T Zögg, J Yin, D Yuan, A Wohlkönig… - Scientific reports, 2019 - nature.com
Fusions to the C-terminal end of the Aga2p mating adhesion of Saccharomyces cerevisiae
have been used in many studies for the selection of affinity reagents by yeast display …

Research progresses and applications of fluorescent protein antibodies: a review focusing on nanobodies

YL Chen, XX **e, N Zhong, LC Sun, D Lin… - International Journal of …, 2023 - mdpi.com
Since the discovery of fluorescent proteins (FPs), their rich fluorescence spectra and
photochemical properties have promoted widespread biological research applications. FPs …

Surface display as a functional screening platform for detecting enzymes active on PET

SAH Heyde, J Arnling Bååth, P Westh… - Microbial Cell …, 2021 - Springer
Abstract Poly (ethylene terephthalate)(PET) is the world's most abundant polyester plastic,
and its ongoing accumulation in nature is causing a global environmental problem …

Nanoscale features of tunable bacterial outer membrane models revealed by correlative microscopy

K Bali, Z Mohamed, A Scheeder, AM Pappa, S Daniel… - Langmuir, 2022 - ACS Publications
The rise of antibiotic resistance is a growing worldwide human health issue, with major
socioeconomic implications. An understanding of the interactions occurring at the bacterial …

[HTML][HTML] Genetically surface-modified Escherichia coli outer membrane vesicles targeting MUC1 antigen in cancer cells

S Laotee, W Arunmanee - Biotechnology Reports, 2024 - Elsevier
Outer membrane vesicles (OMVs), non-replicating spherical liposomes derived from Gram-
negative bacteria, are a promising vaccine platform and multifunctional delivery systems …

Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly

L Zhang, L Tan, M Liu, Y Chen, Y Yang, Y Zhang… - Microbial Cell …, 2024 - Springer
Background Microbial cell surface display technology allows immobilizing proteins on the
cell surface by fusing them to anchoring motifs, thereby endowing the cells with diverse …

Construction of the lithium binding peptide displayed recombinant Escherichia coli for the specific lithium removal from various metal polluted wastewater

V Selvamani, J Jeong, M kannan Maruthamuthu… - Journal of …, 2023 - Elsevier
Considering the immense increase of lithium battery use, the development of efficient and
environmentally friendly lithium recovering process is required. Here, we demonstrate a …

LyGo: a platform for rapid screening of lytic polysaccharide monooxygenase production

C Hernández-Rollán, KB Falkenberg… - ACS Synthetic …, 2021 - ACS Publications
Environmentally friendly sources of energy and chemicals are essential constituents of a
sustainable society. An important step toward this goal is the utilization of biomass to supply …