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Isolation of plant nuclei for estimation of nuclear DNA content: overview and best practices
A critical aspect for obtaining accurate, reliable, and high‐resolution estimates of nuclear
DNA content is the release of nuclei from the cytoplasm in sufficient amounts, while …
DNA content is the release of nuclei from the cytoplasm in sufficient amounts, while …
Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status
L D'hondt, M Höfte, E Van Bockstaele… - Molecular Plant …, 2011 - Wiley Online Library
Flow cytometers are probably the most multipurpose laboratory devices available. They can
analyse a vast and very diverse range of cell parameters. This technique has left its mark on …
analyse a vast and very diverse range of cell parameters. This technique has left its mark on …
Induction and Flow Cytometry Identification of Tetraploids from Seed‐Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don
SH ** of intracellular protein and nucleic acid in femtograms per pixel
MC Cheung, JG Evans, B McKenna… - Cytometry Part …, 2011 - Wiley Online Library
By using imaging spectrophotometry with paired images in the 200‐to 280‐nm wavelength
range, we have directly mapped intracellular nucleic acid and protein distributions across a …
range, we have directly mapped intracellular nucleic acid and protein distributions across a …
The genome size of clusterbean (Cyamopsis tetragonoloba) is significantly smaller compared to its wild relatives as estimated by flow cytometry
Clusterbean (C. tetragonoloba) is an important, leguminous vegetable and industrial crop
with vast genetic diversity but meager genetic, cytological and genomic information. In the …
with vast genetic diversity but meager genetic, cytological and genomic information. In the …
The effects of rapid desiccation on estimates of plant genome size
Flow cytometry has become the dominant method for estimating nuclear DNA content in
plants, either for ploidy determination or quantification of absolute genome size. Current best …
plants, either for ploidy determination or quantification of absolute genome size. Current best …
Nuclear DNA amounts in Chinese bryophytes estimated by flow cytometry: variation patterns and biological significances
D Li, G Luo, S Guo, R Huang, J Yang, T Cao, J Yu - Plants, 2023 - mdpi.com
There exists an obvious gap in our knowledge of the nuclear DNA amount of bryophytes, not
only in terms of the low number of species represented, but also in systematic and …
only in terms of the low number of species represented, but also in systematic and …
Flow cytometry in plant research: a success story
J Vrána, P Cápal, M Bednářová, J Doležel - Applied Plant Cell Biology …, 2014 - Springer
Flow cytometry is a powerful technique with numerous applications in biomedical research,
including immunology, haematology, oncology and other fields. It has also found important …
including immunology, haematology, oncology and other fields. It has also found important …