Factors involved in heterologous expression of proteins in E. coli host
M Pouresmaeil, S Azizi-Dargahlou - Archives of microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …
biotechnology in the last century. These proteins are produced in the eukaryotic or …
[HTML][HTML] Insights on the emerging biotechnology of histidine-rich peptides
In the late 70's, the discovery of the restriction enzymes made possible the biological
production of functional proteins by recombinant DNA technologies, a fact that largely …
production of functional proteins by recombinant DNA technologies, a fact that largely …
Isothermal analysis of ThermoFluor data can readily provide quantitative binding affinities
Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay,
has become a commonly-used approach for detecting protein-ligand interactions …
has become a commonly-used approach for detecting protein-ligand interactions …
In silico designed novel multi-epitope mRNA vaccines against Brucella by targeting extracellular protein BtuB and LptD
J Shi, Y Zhu, Z Yin, Y He, Y Li, G Haimiti, X **e… - Scientific Reports, 2024 - nature.com
Brucella, a gram-negative intracellular bacterium, causing Brucellosis, a zoonotic disease
with a range of clinical manifestations, from asymptomatic to fever, fatigue, loss of appetite …
with a range of clinical manifestations, from asymptomatic to fever, fatigue, loss of appetite …
A comprehensive, flexible collection of SARS-CoV-2 coding regions
The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2
coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS …
coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS …
A systematic review about affinity tags for one-step purification and immobilization of recombinant proteins: integrated bioprocesses aiming both economic and …
The present study reviewed and discussed the promising affinity tags for one-step
purification and immobilization of recombinant proteins. The approach used to structure this …
purification and immobilization of recombinant proteins. The approach used to structure this …
Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in …
M Knödler, P Opdensteinen… - Biotechnology and …, 2023 - Wiley Online Library
Nanoparticles are used as carriers for the delivery of drugs and imaging agents. Proteins are
safer than synthetic nanocarriers due to their greater biocompatibility and the absence of …
safer than synthetic nanocarriers due to their greater biocompatibility and the absence of …
Fabrication of gelatin-incorporated nanoporous chitosan-based membranes for potential water desalination applications
Membrane technology has extensively been used in diverse phenomena such as
separation, purification and controlled transportation. Herein, gelatin-incorporated porous …
separation, purification and controlled transportation. Herein, gelatin-incorporated porous …
Effects of N‐terminal and C‐terminal polyhistidine tag on the stability and function of the thermophilic P450 CYP119
Y Aslantas, NB Surmeli - Bioinorganic chemistry and …, 2019 - Wiley Online Library
Biocatalysts are sought‐after in synthesis of pharmaceuticals and agrochemicals due to their
high regioselectivity and enantioselectivity. Among biocatalysts, heme‐containing …
high regioselectivity and enantioselectivity. Among biocatalysts, heme‐containing …
Preclinical validation of human recombinant glutamate-oxaloacetate transaminase for the treatment of acute ischemic stroke
M Pérez-Mato, A Dopico-López, Y Akkoc… - Iscience, 2024 - cell.com
The blood enzyme glutamate-oxaloacetate transaminase (GOT) has been postulated as an
effective therapeutic to protect the brain during stroke. To demonstrate its potential clinical …
effective therapeutic to protect the brain during stroke. To demonstrate its potential clinical …