RNA m6A methylation across the transcriptome

E Sendinc, Y Shi - Molecular cell, 2023‏ - cell.com
Since the early days of foundational studies of nucleic acids, many chemical moieties have
been discovered to decorate RNA and DNA in diverse organisms. In mammalian cells, one …

Mechanisms of lncRNA biogenesis as revealed by nascent transcriptomics

T Nojima, NJ Proudfoot - Nature reviews Molecular cell biology, 2022‏ - nature.com
Mammalian genomes express two principal gene categories through RNA polymerase II-
mediated transcription: protein-coding transcription units and non-coding RNA transcription …

H3K4me3 regulates RNA polymerase II promoter-proximal pause-release

H Wang, Z Fan, PV Shliaha, M Miele, RC Hendrickson… - Nature, 2023‏ - nature.com
Trimethylation of histone H3 lysine 4 (H3K4me3) is associated with transcriptional start sites
and has been proposed to regulate transcription initiation,. However, redundant functions of …

Structural basis of Integrator-dependent RNA polymerase II termination

I Fianu, M Ochmann, JL Walshe, O Dybkov, JN Cruz… - Nature, 2024‏ - nature.com
The Integrator complex can terminate RNA polymerase II (Pol II) in the promoter-proximal
region of genes. Previous work has shed light on how Integrator binds to the paused …

P-TEFb: The master regulator of transcription elongation

K Fu**aga, F Huang, BM Peterlin - Molecular cell, 2023‏ - cell.com
The positive transcription elongation factor b (P-TEFb) is composed of cyclins T1 or T2 and
cyclin-dependent kinase 9 that regulate the elongation phase of transcription by RNA …

3′-end processing of eukaryotic mRNA: machinery, regulation, and impact on gene expression

V Boreikaitė, LA Passmore - Annual review of biochemistry, 2023‏ - annualreviews.org
Formation of the 3′ end of a eukaryotic mRNA is a key step in the production of a mature
transcript. This process is mediated by a number of protein factors that cleave the pre …

Transcriptional elongation control in developmental gene expression, aging, and disease

Y Aoi, A Shilatifard - Molecular cell, 2023‏ - cell.com
The elongation stage of transcription by RNA polymerase II (RNA Pol II) is central to the
regulation of gene expression in response to developmental and environmental cues in …

R-loop-dependent promoter-proximal termination ensures genome stability

C Xu, C Li, J Chen, Y **ong, Z Qiao, P Fan, C Li, S Ma… - Nature, 2023‏ - nature.com
The proper regulation of transcription is essential for maintaining genome integrity and
executing other downstream cellular functions,. Here we identify a stable association …

Integrator is a global promoter-proximal termination complex

EJ Wagner, L Tong, K Adelman - Molecular cell, 2023‏ - cell.com
Integrator is a metazoan-specific protein complex capable of inducing termination at all
RNAPII-transcribed loci. Integrator recognizes paused, promoter-proximal RNAPII and …

Integrator endonuclease drives promoter-proximal termination at all RNA polymerase II-transcribed loci

CB Stein, AR Field, CA Mimoso, CC Zhao, KL Huang… - Molecular cell, 2022‏ - cell.com
Summary RNA polymerase II (RNAPII) pausing in early elongation is critical for gene
regulation. Paused RNAPII can be released into productive elongation by the kinase P-TEFb …