Computational deconvolution of transcriptomics data from mixed cell populations

F Avila Cobos, J Vandesompele, P Mestdagh… - …, 2018 - academic.oup.com
Gene expression analyses of bulk tissues often ignore cell type composition as an important
confounding factor, resulting in a loss of signal from lowly abundant cell types. In this review …

Transcriptomics for child and adolescent tuberculosis

M Kaforou, C Broderick, O Vito, M Levin… - Immunological …, 2022 - Wiley Online Library
Tuberculosis (TB) in humans is caused by Mycobacterium tuberculosis (Mtb). It is estimated
that 70 million children (< 15 years) are currently infected with Mtb, with 1.2 million each year …

APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids

J Zhao, Y Fu, Y Yamazaki, Y Ren, MD Davis… - Nature …, 2020 - nature.com
APOE4 is the strongest genetic risk factor associated with late-onset Alzheimer's disease
(AD). To address the underlying mechanism, we develop cerebral organoid models using …

Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease

J Park, R Shrestha, C Qiu, A Kondo, S Huang, M Werth… - Science, 2018 - science.org
Our understanding of kidney disease pathogenesis is limited by an incomplete molecular
characterization of the cell types responsible for the organ's multiple homeostatic functions …

Scater: pre-processing, quality control, normalization and visualization of single-cell RNA-seq data in R

DJ McCarthy, KR Campbell, ATL Lun, QF Wills - Bioinformatics, 2017 - academic.oup.com
Motivation Single-cell RNA sequencing (scRNA-seq) is increasingly used to study gene
expression at the level of individual cells. However, preparing raw sequence data for further …

Kidney decellularized extracellular matrix enhanced the vascularization and maturation of human kidney organoids

JW Kim, SA Nam, J Yi, JY Kim, JY Lee… - Advanced …, 2022 - Wiley Online Library
Kidney organoids derived from human pluripotent stem cells (hPSCs) have extensive
potential for disease modelling and regenerative medicine. However, the limited …

Brain cell type specific gene expression and co-expression network architectures

AT McKenzie, M Wang, ME Hauberg, JF Fullard… - Scientific reports, 2018 - nature.com
Elucidating brain cell type specific gene expression patterns is critical towards a better
understanding of how cell-cell communications may influence brain functions and …

The role of m6A/m-RNA methylation in stress response regulation

M Engel, C Eggert, PM Kaplick, M Eder, S Röh, L Tietze… - Neuron, 2018 - cell.com
Summary N 6-methyladenosine (m 6 A) and N 6, 2′-O-dimethyladenosine (m 6 Am) are
abundant mRNA modifications that regulate transcript processing and translation. The role …

Treg cells promote the SREBP1-dependent metabolic fitness of tumor-promoting macrophages via repression of CD8+ T cell-derived interferon-γ

C Liu, M Chikina, R Deshpande, AV Menk, T Wang… - Immunity, 2019 - cell.com
Regulatory T (Treg) cells are crucial for immune homeostasis, but they also contribute to
tumor immune evasion by promoting a suppressive tumor microenvironment (TME). Mice …

The nuclear receptor ESRRA protects from kidney disease by coupling metabolism and differentiation

P Dhillon, J Park, CH Del Pozo, L Li, T Doke, S Huang… - Cell metabolism, 2021 - cell.com
Kidney disease is poorly understood because of the organ's cellular diversity. We used
single-cell RNA sequencing not only in resolving differences in injured kidney tissue cellular …