Substrate channelling as an approach to cascade reactions
Millions of years of evolution have produced biological systems capable of efficient one-pot
multi-step catalysis. The underlying mechanisms that facilitate these reaction processes are …
multi-step catalysis. The underlying mechanisms that facilitate these reaction processes are …
Engineering enzyme microenvironments for enhanced biocatalysis
L Lancaster, W Abdallah, S Banta… - Chemical Society …, 2018 - pubs.rsc.org
Protein engineering provides a means to alter protein structure leading to new functions.
Much work has focused on the engineering of enzyme active sites to enhance catalytic …
Much work has focused on the engineering of enzyme active sites to enhance catalytic …
Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion
Cells routinely compartmentalize enzymes for enhanced efficiency of their metabolic
pathways. Here we report a general approach to construct DNA nanocaged enzymes for …
pathways. Here we report a general approach to construct DNA nanocaged enzymes for …
Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades
Access to efficient enzymatic channeling is desired for improving all manner of designer
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …
[HTML][HTML] Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade
A proximity effect has been invoked to explain the enhanced activity of enzyme cascades on
DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish …
DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish …
Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress
Artificial multienzyme scaffolds are being developed for in vitro cascaded biocatalytic activity
and, in particular, accessing substrate channeling. This review covers progress in this field …
and, in particular, accessing substrate channeling. This review covers progress in this field …
Toward rational design of high-efficiency enzyme cascades
The vision of assembling molecules in a manner similar to a factory, where parts pass from
workstation to workstation, is highly appealing. A key requirement to achieve such control …
workstation to workstation, is highly appealing. A key requirement to achieve such control …
Tailoring enzyme microenvironment: State-of-the-art strategy to fulfill the quest for efficient bio-catalysis
Enzymes as green industrial biocatalysts have become a powerful norm that offers several
advantages over traditional catalytic agents with regard to process efficiency, reusability …
advantages over traditional catalytic agents with regard to process efficiency, reusability …
Increasing enzyme cascade throughput by pH-engineering the microenvironment of individual enzymes
The throughput of enzyme cascade reactions could be increased if the environmental
conditions could be optimized for each enzyme in the cascade individually. Here, we …
conditions could be optimized for each enzyme in the cascade individually. Here, we …
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis
Natural cascades frequently use spatial organization to introduce beneficial substrate
channeling mechanisms, a strategy that has been widely mimicked in many engineered …
channeling mechanisms, a strategy that has been widely mimicked in many engineered …