Illuminating RNA biology: tools for imaging RNA in live mammalian cells
The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the main
building blocks of the cell. But this over simplified linear transmission of information …
building blocks of the cell. But this over simplified linear transmission of information …
Advances in fluorescence and bioluminescence imaging
Recent progress of bioanalytical techniques has accelerated the deep understanding of
cellular states and development of novel drugs as well as medical diagnosis. Particularly …
cellular states and development of novel drugs as well as medical diagnosis. Particularly …
Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes
RNA plays an important role in life processes. Imaging of messenger RNAs (mRNAs) and
micro-RNAs (miRNAs) not only allows us to learn the formation and transcription of mRNAs …
micro-RNAs (miRNAs) not only allows us to learn the formation and transcription of mRNAs …
Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation
Genome editing has enabled broad advances and novel approaches in studies of gene
function and structure; now, emerging methods aim to precisely engineer post …
function and structure; now, emerging methods aim to precisely engineer post …
Modulation of miRNA function by natural and synthetic RNA-binding proteins in cancer
RNA-binding proteins (RBPs) and microRNAs (miRNAs) are the most important regulators of
mRNA stability and translation in eukaryotic cells; however, the complex interplay between …
mRNA stability and translation in eukaryotic cells; however, the complex interplay between …
Fluorescence chemosensors for hydrogen sulfide detection in biological systems
Z Guo, G Chen, G Zeng, Z Li, A Chen, J Wang, L Jiang - Analyst, 2015 - pubs.rsc.org
A comprehensive review of the development of H2S fluorescence-sensing strategies,
including sensors based on chemical reactions and fluorescence resonance energy transfer …
including sensors based on chemical reactions and fluorescence resonance energy transfer …
Programmable RNA-binding protein composed of repeats of a single modular unit
KP Adamala, DA Martin-Alarcon… - Proceedings of the …, 2016 - National Acad Sciences
The ability to monitor and perturb RNAs in living cells would benefit greatly from a modular
protein architecture that targets unmodified RNA sequences in a programmable way. We …
protein architecture that targets unmodified RNA sequences in a programmable way. We …
Split luciferase-fragment reconstitution for unveiling RNA localization and dynamics in live cells
The intracellular distribution and dynamics of RNAs play pivotal roles in various
physiological phenomena. The ability to monitor the amount and localization of endogenous …
physiological phenomena. The ability to monitor the amount and localization of endogenous …
[PDF][PDF] Nanoscale dynamics and localization of single endogenous mRNAs in stress granules
K Sugawara, S Uno, M Kamiya… - Nucleic Acids …, 2024 - academic.oup.com
Stress granules (SGs) are cytoplasmic messenger ribonucleoprotein granules transiently
formed in stressed mammalian cells. Although SG components have been well …
formed in stressed mammalian cells. Although SG components have been well …
Single molecule fluorescence approaches shed light on intracellular RNAs
S Pitchiaya, LA Heinicke, TC Custer… - Chemical reviews, 2014 - ACS Publications
The eukaryotic cell is highly complex. Ever since Robert Hooke discovered “cells” in 1665
when training his comparably primitive microscope on a sliver of cork, scientists have aimed …
when training his comparably primitive microscope on a sliver of cork, scientists have aimed …