Nitric oxide (NO) is an important signaling molecule that is involved in a wide range of
physiological and pathological events in biology. Metal coordination chemistry, especially …

With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

BACKGROUND Conformational changes are essential for the correct functioning of
biological macromolecules. Time-resolved x-ray crystallography extends an extremely …

Highlights•Time-resolved serial femtosecond crystallography (tr-SFX) probes structure and
function in each sample.•X-ray free electron lasers (XFELS) provide atomic and electronic …

In this review, we describe recent research developments into radiation damage effects in
macromolecular X-ray crystallography observed at synchrotrons and X-ray free electron …

Careful selection of photocaging approaches is critical to achieve fast and well synchronized
reaction initiation and perform successful time-resolved structural biology experiments. This …

Highly efficient data-collection methods are required for successful macromolecular
crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is …

Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to
control neural activity with light. Light absorption causes retinal chromophore isomerization …

Serial femtosecond crystallography has opened up many new opportunities in structural
biology. In recent years, several approaches employing light-inducible systems have …

Protein structural biology came a long way since the determination of the first three-
dimensional structure of myoglobin about six decades ago. Across this period, X-ray …