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Generation of allogenic chimera carrying mutations in PDX1 and TP53 genes via phytohemagglutinin-mediated blastomere aggregation in pigs
TV Nguyen, K Takebayashi, LTK Do, Z Namula… - In Vitro Cellular & …, 2024 - Springer
The generation of genetically engineered pig models that develop pancreas-specific tumors
has the potential to advance studies and our understanding of pancreatic cancer in humans …
has the potential to advance studies and our understanding of pancreatic cancer in humans …
Slow freezing cryopreservation of Korean bovine blastocysts with an additional sucrose pre-equilibration step
Introduction Embryo cryopreservation is a valuable technique used for preserving genetic
resources for long periods. However, the survival rate of embryos is dependent on the …
resources for long periods. However, the survival rate of embryos is dependent on the …
GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development
G Heo, SH Lee, JD Kim, GH Lee, JM Sim… - The FASEB …, 2023 - Wiley Online Library
Abstract Glucose‐regulated protein 78 (GRP78) binds to and stabilizes melanocortin 4
receptor (MC4R), which activates protein kinase A (PKA) by regulating G proteins. GRP78 is …
receptor (MC4R), which activates protein kinase A (PKA) by regulating G proteins. GRP78 is …
Optimization of embryonic stage for aggregation to generate chimeric pigs using gene-edited blastomeres
K Takebayashi, M Wittayarat, M Hirata, Q Lin… - In Vitro Cellular & …, 2024 - Springer
The high mortality rate and increasing incidence of cancer are major issues (Bray et al.
2018, Chowdhury et al. 2020). Pigs show anatomical/physiological characteristics to those …
2018, Chowdhury et al. 2020). Pigs show anatomical/physiological characteristics to those …
Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs
Tetraploid complementation is an ideal method for demonstrating the differentiation potential
of pluripotent stem cells. In this study, we selected the most efficient tetraploid production …
of pluripotent stem cells. In this study, we selected the most efficient tetraploid production …
Developmental competence of chimeric porcine embryos through the aggregation of parthenogenetic embryos and somatic cell nuclear transfer embryos
The efficiency of somatic cell nuclear transfer (NT) in pigs is low and requires enhancement.
We identified the most efficient method for zona pellucida (ZP) removal and blastomere …
We identified the most efficient method for zona pellucida (ZP) removal and blastomere …