Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

Abstract The 4D Nucleome Network aims to develop and apply approaches to map the
structure and dynamics of the human and mouse genomes in space and time with the goal …

DNA double-strand breaks repaired by non-homologous end joining display limited DNA
end-processing and chromosomal mobility. By contrast, double-strand breaks undergoing …

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …

The spatiotemporal organization and dynamics of chromatin play critical roles in regulating
genome function. However, visualizing specific, endogenous genomic loci remains …

Enhancers are important regulatory elements that can control gene activity across vast
genetic distances. However, the underlying nature of this regulation remains obscured …

To achieve guide RNA (gRNA) multiplexing and an efficient delivery of tens of distinct
gRNAs into single cells, we developed a molecular assembly strategy termed chimeric array …

The primary regulators of metazoan gene expression are enhancers, originally functionally
defined as DNA sequences that can activate transcription at promoters in an orientation …

In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a
homologous DNA sequence (ie, homologous recombination [HR]), focusing mainly on yeast …