Phase transitions of associative biomacromolecules

RV Pappu, SR Cohen, F Dar, M Farag, M Kar - Chemical Reviews, 2023 - ACS Publications
Multivalent proteins and nucleic acids, collectively referred to as multivalent associative
biomacromolecules, provide the driving forces for the formation and compositional …

The molecular basis for cellular function of intrinsically disordered protein regions

AS Holehouse, BB Kragelund - Nature Reviews Molecular Cell Biology, 2024 - nature.com
Intrinsically disordered protein regions exist in a collection of dynamic interconverting
conformations that lack a stable 3D structure. These regions are structurally heterogeneous …

Phase separation of protein mixtures is driven by the interplay of homotypic and heterotypic interactions

M Farag, WM Borcherds, A Bremer, T Mittag… - Nature …, 2023 - nature.com
Prion-like low-complexity domains (PLCDs) are involved in the formation and regulation of
distinct biomolecular condensates that form via phase separation coupled to percolation …

Condensates formed by prion-like low-complexity domains have small-world network structures and interfaces defined by expanded conformations

M Farag, SR Cohen, WM Borcherds, A Bremer… - Nature …, 2022 - nature.com
Biomolecular condensates form via coupled associative and segregative phase transitions
of multivalent associative macromolecules. Phase separation coupled to percolation is one …

Improved predictions of phase behaviour of intrinsically disordered proteins by tuning the interaction range

G Tesei, K Lindorff-Larsen - Open Research Europe, 2023 - pmc.ncbi.nlm.nih.gov
The formation and viscoelastic properties of condensates of intrinsically disordered proteins
(IDPs) is dictated by amino acid sequence and solution conditions. Because of the …

Molecular and environmental determinants of biomolecular condensate formation

JA Villegas, M Heidenreich, ED Levy - Nature chemical biology, 2022 - nature.com
Biomolecular condensate formation has been implicated in a host of biological processes
and has found relevance in biology and disease. Understanding the physical principles and …

Aging can transform single-component protein condensates into multiphase architectures

A Garaizar, JR Espinosa, JA Joseph… - Proceedings of the …, 2022 - National Acad Sciences
Phase-separated biomolecular condensates that contain multiple coexisting phases are
widespread in vitro and in cells. Multiphase condensates emerge readily within …

Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates

I Alshareedah, WM Borcherds, SR Cohen, A Singh… - Nature Physics, 2024 - nature.com
Biomolecular condensates are viscoelastic materials. Here we investigate the determinants
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …

Design of intrinsically disordered protein variants with diverse structural properties

F Pesce, A Bremer, G Tesei, JB Hopkins, CR Grace… - Science …, 2024 - science.org
Intrinsically disordered proteins (IDPs) perform a broad range of functions in biology,
suggesting that the ability to design IDPs could help expand the repertoire of proteins with …

Adenosine triphosphate mediates phase separation of disordered basic proteins by bridging intermolecular interaction networks

D Kota, R Prasad, HX Zhou - Journal of the American Chemical …, 2024 - ACS Publications
Adenosine triphosphate (ATP) is an abundant molecule with crucial cellular roles as the
energy currency and a building block of nucleic acids and for protein phosphorylation. Here …