Optics-integrated microfluidic platforms for biomolecular analyses

KE Bates, H Lu - Biophysical journal, 2016 - cell.com
Compared with conventional optical methods, optics implemented on microfluidic chips
provide small, and often much cheaper ways to interrogate biological systems from the level …

Numerical–experimental observation of shape bistability of red blood cells flowing in a microchannel

A Guckenberger, A Kihm, T John, C Wagner, S Gekle - Soft Matter, 2018 - pubs.rsc.org
Red blood cells flowing through capillaries assume a wide variety of different shapes owing
to their high deformability. Predicting the realized shapes is a complex field as they are …

Microarray expression profiling and raman spectroscopy reveal anti-fatty liver action of berberine in a diet-induced larval zebrafish model

B Chen, YM Zheng, MQ Zhang, Y Han… - Frontiers in …, 2020 - frontiersin.org
Background: The prevalence of non-alcohol fatty liver disease (NAFLD) is increasing in
children and adolescents who are mostly resulted from overfeeding. Previous studies …

Flow-scanning optical tomography

NC Pégard, ML Toth, M Driscoll, JW Fleischer - Lab on a Chip, 2014 - pubs.rsc.org
We present a 3D tomography technique for in vivo observation of microscopic samples. The
method combines flow in a microfluidic channel, illumination through a slit aperture, and a …

Slanted channel microfluidic chip for 3D fluorescence imaging of cells in flow

VK Jagannadh, MD Mackenzie, P Pal, AK Kar… - Optics express, 2016 - opg.optica.org
Three-dimensional cellular imaging techniques have become indispensable tools in
biological research and medical diagnostics. Conventional 3D imaging approaches employ …

Imaging flow cytometry with femtosecond laser-micromachined glass microfluidic channels

VK Jagannadh, MD Mackenzie, P Pal… - IEEE Journal of …, 2014 - ieeexplore.ieee.org
Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing
properties of cells/particles while they are in flow. In this paper, we demonstrate the …

Framework for morphometric classification of cells in imaging flow cytometry

G Gopakumar, VK Jagannadh, SS Gorthi… - Journal of …, 2016 - Wiley Online Library
Imaging flow cytometry is an emerging technology that combines the statistical power of flow
cytometry with spatial and quantitative morphology of digital microscopy. It allows high …

Automated cell viability assessment using a microfluidics based portable imaging flow analyzer

VK Jagannadh, JV Adhikari, SS Gorthi - Biomicrofluidics, 2015 - pubs.aip.org
In this work, we report a system-level integration of portable microscopy and microfluidics for
the realization of optofluidic imaging flow analyzer with a throughput of 450 cells/s. With the …

High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry

VK Jagannadh, BP Bhat, LA Nirupa Julius… - Analytical and …, 2016 - Springer
In this article, we present a novel approach to throughput enhancement in miniaturized
microfluidic microscopy systems. Using the presented approach, we demonstrate an …

Field-portable microfluidics-based imaging flow cytometer

VK Jagannadh, RS Murthy, R Srinivasan… - Journal of Lightwave …, 2015 - opg.optica.org
Clinical microscopy is a versatile and robust tool used for the diagnosis of a plethora of
diseases. However, due to various reasons, it remains inaccessible in resource limited …