A conceptual framework for understanding phase separation and addressing open questions and challenges
Macromolecular phase separation is being recognized for its potential importance and
relevance as a driver of spatial organization within cells. Here, we describe a framework …
relevance as a driver of spatial organization within cells. Here, we describe a framework …
Formation of chromatin subcompartments by phase separation
Chromatin is partitioned on multiple length scales into subcompartments that differ from
each other with respect to their molecular composition and biological function. It is a key …
each other with respect to their molecular composition and biological function. It is a key …
Condensate interfacial forces reposition DNA loci and probe chromatin viscoelasticity
Biomolecular condensates assemble in living cells through phase separation and related
phase transitions. An underappreciated feature of these dynamic molecular assemblies is …
phase transitions. An underappreciated feature of these dynamic molecular assemblies is …
[HTML][HTML] The 4D nucleome: Evidence for a dynamic nuclear landscape based on co-aligned active and inactive nuclear compartments
Recent methodological advancements in microscopy and DNA sequencing-based methods
provide unprecedented new insights into the spatio-temporal relationships between …
provide unprecedented new insights into the spatio-temporal relationships between …
The interchromatin compartment participates in the structural and functional organization of the cell nucleus
This article focuses on the role of the interchromatin compartment (IC) in sha** nuclear
landscapes. The IC is connected with nuclear pore complexes (NPCs) and harbors splicing …
landscapes. The IC is connected with nuclear pore complexes (NPCs) and harbors splicing …
Correlating nuclear morphology and external force with combined atomic force microscopy and light sheet imaging separates roles of chromatin and lamin A/C in …
CM Hobson, M Kern, ET O'Brien III… - Molecular biology of …, 2020 - Am Soc Cell Biol
Nuclei are often under external stress, be it during migration through tight constrictions or
compressive pressure by the actin cap, and the mechanical properties of nuclei govern their …
compressive pressure by the actin cap, and the mechanical properties of nuclei govern their …
The rich inner life of the cell nucleus: dynamic organization, active flows, and emergent rheology
A Zidovska - Biophysical Reviews, 2020 - Springer
The cell nucleus stores the genetic material essential for life, and provides the environment
for transcription, maintenance, and replication of the genome. Moreover, the nucleoplasm is …
for transcription, maintenance, and replication of the genome. Moreover, the nucleoplasm is …
[HTML][HTML] Dealing with the nucleus during cell migration
FJ Calero-Cuenca, CS Janota, ER Gomes - Current opinion in cell biology, 2018 - Elsevier
The position of the nucleus within cells is a key event during cell migration. The movement
and positioning of the nucleus strongly impacts cell migration. Notably, the last two years …
and positioning of the nucleus strongly impacts cell migration. Notably, the last two years …
[HTML][HTML] Repetitive RNAs as regulators of chromatin-associated subcompartment formation by phase separation
Repetitive RNA (repRNA) sequences emerge as important regulators of the dynamic
organization of genomic loci into membrane-less subcompartments with distinct nuclear …
organization of genomic loci into membrane-less subcompartments with distinct nuclear …
A survey of physical methods for studying nuclear mechanics and mechanobiology
It is increasingly appreciated that the cell nucleus is not only a home for DNA but also a
complex material that resists physical deformations and dynamically responds to external …
complex material that resists physical deformations and dynamically responds to external …