Cutting back malaria: CRISPR/Cas9 genome editing of Plasmodium

MCS Lee, SE Lindner, JJ Lopez-Rubio… - Briefings in functional …, 2019 - academic.oup.com
CRISPR/Cas9 approaches are revolutionizing our ability to perform functional genomics
across a wide range of organisms, including the Plasmodium parasites that cause malaria …

[HTML][HTML] Therapeutic and diagnostic relevance of Crispr technology

A Djekoun - Biomedicine & Pharmacotherapy, 2021 - Elsevier
CRISPR is a family of DNA repeats providing immunity against viral and plasmid invading
DNA in bacteria and archaea. The system consist of an endonuclease Cas, guided by a …

Improvement of CRISPR/Cas9 system by transfecting Cas9-expressing Plasmodium berghei with linear donor template

N Shinzawa, T Nishi, F Hiyoshi, D Motooka… - Communications …, 2020 - nature.com
Malaria is caused by infection with Plasmodium parasites and is a major public health
concern. The CRISPR/Cas9 system is a promising technology, but still has technical …

Creation and preclinical evaluation of genetically attenuated malaria parasites arresting growth late in the liver

B Franke-Fayard, C Marin-Mogollon, FJA Geurten… - npj Vaccines, 2022 - nature.com
Whole-sporozoite (WSp) malaria vaccines induce protective immune responses in animal
malaria models and in humans. A recent clinical trial with a WSp vaccine comprising …

Highly efficient CRISPR/Cas9 system in Plasmodium falciparum using Cas9-expressing parasites and a linear donor template

T Nishi, N Shinzawa, M Yuda, S Iwanaga - Scientific reports, 2021 - nature.com
The CRISPR/Cas9 system is a powerful genetic engineering technology for Plasmodium
falciparum. We here report further improvement of the CRISPR/Cas9 system by combining …

Genetic manipulation toolkits in Apicomplexan parasites

Z Niu, L Xue, X Yin, B Shen - Zoonoses, 2022 - scienceopen.com
Apicomplexan parasites are a group of intracellular pathogens of great medical and
veterinary importance, including Toxoplasma gondii and Plasmodium, which cause …