Analysing microbial community composition through amplicon sequencing: from sampling to hypothesis testing
Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …
past decade's revolution in DNA sequencing cost and throughput has made it possible for …
Using metagenomics to investigate human and environmental resistomes
Antibiotic resistance is a global health concern declared by the WHO as one of the largest
threats to modern healthcare. In recent years, metagenomic DNA sequencing has started to …
threats to modern healthcare. In recent years, metagenomic DNA sequencing has started to …
Metagenomic profiling pipelines improve taxonomic classification for 16S amplicon sequencing data
Most experiments studying bacterial microbiomes rely on the PCR amplification of all or part
of the gene for the 16S rRNA subunit, which serves as a biomarker for identifying and …
of the gene for the 16S rRNA subunit, which serves as a biomarker for identifying and …
Temporal variability in soil microbial communities across land-use types
Although numerous studies have investigated changes in soil microbial communities across
space, questions about the temporal variability in these communities and how this variability …
space, questions about the temporal variability in these communities and how this variability …
Colonization and succession within the human gut microbiome by archaea, bacteria, and microeukaryotes during the first year of life
L Wampach, A Heintz-Buschart, A Hogan… - Frontiers in …, 2017 - frontiersin.org
Perturbations to the colonization process of the human gastrointestinal tract have been
suggested to result in adverse health effects later in life. Although much research has been …
suggested to result in adverse health effects later in life. Although much research has been …
Bacterial diversity along a 2600 km river continuum
The bacterioplankton diversity in large rivers has thus far been under‐sampled despite the
importance of streams and rivers as components of continental landscapes. Here, we …
importance of streams and rivers as components of continental landscapes. Here, we …
Assessing ecological status with diatoms DNA metabarcoding: Scaling-up on a WFD monitoring network (Mayotte island, France)
Diatoms are excellent ecological indicators of water quality because they are broadly
distributed, they show high species diversity and they respond rapidly to human pressures …
distributed, they show high species diversity and they respond rapidly to human pressures …
Microdiversity ensures the maintenance of functional microbial communities under changing environmental conditions
Microdiversity can lead to different ecotypes within the same species. These are assumed to
provide stability in time and space to those species. However, the role of microdiversity in …
provide stability in time and space to those species. However, the role of microdiversity in …
Chemical and microbial diversity covary in fresh water to influence ecosystem functioning
Invisible to the naked eye lies a tremendous diversity of organic molecules and organisms
that make major contributions to important biogeochemical cycles. However, how the …
that make major contributions to important biogeochemical cycles. However, how the …
Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling
S Reitmeier, TCA Hitch, N Treichel, N Fikas… - ISME …, 2021 - academic.oup.com
Abstract 16S rRNA gene amplicon sequencing is a popular approach for studying
microbiomes. However, some basic concepts have still not been investigated …
microbiomes. However, some basic concepts have still not been investigated …