[HTML][HTML] Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity

PF Thomsen, E Willerslev - Biological conservation, 2015 - Elsevier
The continuous decline in Earth's biodiversity represents a major crisis and challenge for the
21st century, and there is international political agreement to slow down or halt this decline …

Fungal community analysis by high‐throughput sequencing of amplified markers–a user's guide

BD Lindahl, RH Nilsson, L Tedersoo… - New …, 2013 - Wiley Online Library
Novel high‐throughput sequencing methods outperform earlier approaches in terms of
resolution and magnitude. They enable identification and relative quantification of …

Improved software detection and extraction of ITS1 and ITS 2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing …

J Bengtsson‐Palme, M Ryberg… - Methods in ecology …, 2013 - Wiley Online Library
The nuclear ribosomal internal transcribed spacer (ITS) region is the primary choice for
molecular identification of fungi. Its two highly variable spacers (ITS 1 and ITS 2) are usually …

[PDF][PDF] Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

L Tedersoo, S Anslan, M Bahram, S Põlme, T Riit… - …, 2015 - mycokeys.pensoft.net
Rapid development of high-throughput (HTS) molecular identification methods has
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …

The sooty moulds

P Chomnunti, S Hongsanan, B Aguirre-Hudson, Q Tian… - Fungal diversity, 2014 - Springer
Sooty moulds are a remarkable, but poorly understood group of fungi. They coat fruits and
leaves superficially with black mycelia, which reduces photosynthesis rates of host plants …

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies

M Op De Beeck, B Lievens, P Busschaert, S Declerck… - PloS one, 2014 - journals.plos.org
Current metabarcoding studies aiming to characterize microbial communities generally rely
on the amplification and sequencing of relatively short DNA regions. For fungi, the internal …

Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding

H Krehenwinkel, M Wolf, JY Lim, AJ Rominger… - Scientific reports, 2017 - nature.com
Amplicon based metabarcoding promises rapid and cost-efficient analyses of species
composition. However, it is disputed whether abundance estimates can be derived from …

Forgotten fungi—the gut mycobiome in human health and disease

CE Huseyin, PW O'Toole, PD Cotter… - FEMS Microbiology …, 2017 - academic.oup.com
The human body is home to a complex and diverse microbial ecosystem that plays a central
role in host health. This includes a diversity of fungal species that is collectively referred to …

The online database MaarjAM reveals global and ecosystemic distribution patterns in arbuscular mycorrhizal fungi (Glomeromycota)

M Öpik, A Vanatoa, E Vanatoa, M Moora… - New …, 2010 - Wiley Online Library
Here, we describe a new database, MaarjAM, that summarizes publicly available
Glomeromycota DNA sequence data and associated metadata. The goal of the database is …

454 Pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity

M Buée, M Reich, C Murat, E Morin… - New …, 2009 - Wiley Online Library
Soil fungi play a major role in ecological and biogeochemical processes in forests. Little is
known, however, about the structure and richness of different fungal communities and the …