Plasma membrane proteins have important roles in transport and signal transduction.
Deciphering the spatiotemporal organization of these proteins provides crucial information …

Pathologic α-synuclein plays an important role in the pathogenesis of α-synucleinopathies
such as Parkinson's disease (PD). Disruption of proteostasis is thought to be central to …

In this study, we elucidated the architectures of two multisubunit complexes, the BBSome
and exocyst, through a novel application of fluorescent fusion proteins. By processing …

Proteins execute numerous cell functions in concert with one another in protein–protein
interactions (PPI). While essential in each cell, such interactions are not identical from cell to …

Single-molecule characterization of protein–DNA dynamics provides unprecedented
mechanistic details about numerous nuclear processes. Here, we describe a new method …

The formation of soluble α-synuclein (α-syn) and amyloid-β (Aβ) aggregates is associated
with the development of Parkinson's disease (PD). Current methods mainly focus on the …

Preventing nonspecific binding is essential for sensitive surface-based quantitative single-
molecule microscopy. Here we report a much-simplified RainX-F127 (RF-127) surface with …

DNA and protein microarrays are a high-throughput technology that allow the simultaneous
quantification of tens of thousands of different biomolecular species. The mediocre …

We developed in situ single cell pull-down (SiCPull) of GFP-tagged protein complexes
based on micropatterned functionalized surface architectures. Cells cultured on these …

Highlights•Native complexes are subjected to dynamic spatial and temporal regulation in
cells.•Pulling-down single complexes from cell extract allows real-time observation of …