Colistin regained global interest as a consequence of the rising prevalence of multidrug-
resistant Gram-negative Enterobacteriaceae. In parallel, colistin-resistant bacteria emerged …

Infectious disease outbreaks have caused thousands of deaths and hospitalizations, along
with severe negative global economic impacts. Among these, infections caused by …

Antimicrobial resistance (AMR) is one of the most important health threats globally. The
ability to accurately identify resistant bacterial isolates and the individual antimicrobial …

Objectives WGS-based antimicrobial susceptibility testing (AST) is as reliable as phenotypic
AST for several antimicrobial/bacterial species combinations. However, routine use of WGS …

Background As the cost of sequencing has declined, clinical diagnostics based on next
generation sequencing (NGS) have become reality. Diagnostics based on sequencing will …

The recent advancements in rapid and affordable DNA sequencing technologies have
revolutionized diagnostic microbiology and microbial surveillance. The availability of …

Background Antibiotic resistance is a major health problem, as drugs that were once highly
effective no longer cure bacterial infections. WGS has previously been shown to be an …

Background With the efficiency and the decreasing cost of next-generation sequencing, the
technology is being rapidly introduced into clinical and public health laboratory practice …

Clinical microbiology has long relied on growing bacteria in culture to determine
antimicrobial susceptibility profiles, but the use of whole-genome sequencing for antibiotic …

Outbreaks of multidrug-resistant bacteria present a frequent threat to vulnerable patient
populations in hospitals around the world. Intensive care unit (ICU) patients are particularly …