Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

Two-pore domain K+(K 2P) channels give rise to leak (also called background) K+ currents.
The well-known role of background K+ currents is to stabilize the negative resting …

Two different methods of presenting quantitative gene expression exist: absolute and
relative quantification. Absolute quantification calculates the copy number of the gene …

Background The 2-ΔΔ CT method has been extensively used as a relative quantification
strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This …

The stability of standard gene expression is an elementary prerequisite for internal
standardisation of target gene expression data and many so called housekee** genes …

Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the
product formation with the fluorescent dye SYBR Green I is being extensively used in …

Background Even though real-time PCR has been broadly applied in biomedical sciences,
data processing procedures for the analysis of quantitative real-time PCR are still lacking; …

This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …

Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of
choice for rapid, sensitive, quantitative comparison of RNA transcript abundance. Useful …

Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …