Two-photon calcium imaging of neuronal activity

C Grienberger, A Giovannucci, W Zeiger… - Nature Reviews …, 2022‏ - nature.com
In vivo two-photon calcium imaging (2PCI) is a technique used for recording neuronal
activity in the intact brain. It is based on the principle that, when neurons fire action …

Novel electrode technologies for neural recordings

G Hong, CM Lieber - Nature Reviews Neuroscience, 2019‏ - nature.com
Neural recording electrode technologies have contributed considerably to neuroscience by
enabling the extracellular detection of low-frequency local field potential oscillations and …

High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy

J Demas, J Manley, F Tejera, K Barber, H Kim… - Nature …, 2021‏ - nature.com
Two-photon microscopy has enabled high-resolution imaging of neuroactivity at depth within
scattering brain tissue. However, its various realizations have not overcome the tradeoffs …

Rastermap: a discovery method for neural population recordings

C Stringer, L Zhong, A Syeda, F Du, M Kesa… - Nature …, 2025‏ - nature.com
Neurophysiology has long progressed through exploratory experiments and chance
discoveries. Anecdotes abound of researchers listening to spikes in real time and noticing …

Rapid detection of neurons in widefield calcium imaging datasets after training with synthetic data

Y Zhang, G Zhang, X Han, J Wu, Z Li, X Li, G **ao… - Nature …, 2023‏ - nature.com
Widefield microscopy can provide optical access to multi-millimeter fields of view and
thousands of neurons in mammalian brains at video rate. However, tissue scattering and …

Three-photon neuronal imaging in deep mouse brain

T Wang, C Xu - Optica, 2020‏ - opg.optica.org
Three-photon microscopy has been increasingly adopted for probing neural activities
beyond the typical two-photon imaging depth. In this review, we outline the unique …

High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy

L Streich, JC Boffi, L Wang, K Alhalaseh, M Barbieri… - Nature …, 2021‏ - nature.com
Multiphoton microscopy has become a powerful tool with which to visualize the morphology
and function of neural cells and circuits in the intact mammalian brain. However, tissue …

Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

J Wu, Y Liang, S Chen, CL Hsu, M Chavarha… - Nature …, 2020‏ - nature.com
Understanding information processing in the brain requires monitoring neuronal activity at
high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope …

Video-rate imaging of biological dynamics at centimetre scale and micrometre resolution

J Fan, J Suo, J Wu, H **e, Y Shen, F Chen, G Wang… - Nature …, 2019‏ - nature.com
Large-scale imaging of biological dynamics with high spatiotemporal resolution is
indispensable to system biology studies. However, conventional microscopes have an …

Not so spontaneous: Multi-dimensional representations of behaviors and context in sensory areas

L Avitan, C Stringer - Neuron, 2022‏ - cell.com
Sensory areas are spontaneously active in the absence of sensory stimuli. This
spontaneous activity has long been studied; however, its functional role remains largely …