CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 (Cas9) system has become a successful and promising technology for gene …
protein 9 (Cas9) system has become a successful and promising technology for gene …
CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
The CRISPR–Cas system is a powerful genome editing tool that functions in a diverse array
of organisms and cell types. The technology was initially developed to induce targeted …
of organisms and cell types. The technology was initially developed to induce targeted …
Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Highly specific Cas9 nucleases derived from SpCas9 are valuable tools for genome editing,
but their wide applications are hampered by a lack of knowledge governing guide RNA …
but their wide applications are hampered by a lack of knowledge governing guide RNA …
Pervasive functional translation of noncanonical human open reading frames
Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of
canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based …
canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based …
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …
CRISPR for crop improvement: an update review
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …
approaches has opened up possibilities to breed for almost any given desirable trait …
Delivering CRISPR: a review of the challenges and approaches
CA Lino, JC Harper, JP Carney, JA Timlin - Drug delivery, 2018 - Taylor & Francis
Gene therapy has long held promise to correct a variety of human diseases and defects.
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR
Background The success of the CRISPR/Cas9 genome editing technique depends on the
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
choice of the guide RNA sequence, which is facilitated by various websites. Despite the …
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …
target mutations. Existing strategies for reducing genome-wide off-target effects of the widely …