Current best practices in single‐cell RNA‐seq analysis: a tutorial

MD Luecken, FJ Theis - Molecular systems biology, 2019 - embopress.org
Single‐cell RNA‐seq has enabled gene expression to be studied at an unprecedented
resolution. The promise of this technology is attracting a growing user base for single‐cell …

Orchestrating single-cell analysis with Bioconductor

RA Amezquita, ATL Lun, E Becht, VJ Carey… - Nature …, 2020 - nature.com
Recent technological advancements have enabled the profiling of a large number of
genome-wide features in individual cells. However, single-cell data present unique …

Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid

A Drieu, S Du, SE Storck, J Rustenhoven… - Nature, 2022 - nature.com
Macrophages are important players in the maintenance of tissue homeostasis. Perivascular
and leptomeningeal macrophages reside near the central nervous system (CNS) …

Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma

A Cugurra, T Mamuladze, J Rustenhoven, T Dykstra… - Science, 2021 - science.org
INTRODUCTION Myeloid cells are highly heterogeneous, with distinct ontogenies and
immune functions. The diverse role of myeloid populations, including monocytes …

[HTML][HTML] Functional characterization of the dural sinuses as a neuroimmune interface

J Rustenhoven, A Drieu, T Mamuladze, KA de Lima… - Cell, 2021 - cell.com
Despite the established dogma of central nervous system (CNS) immune privilege,
neuroimmune interactions play an active role in diverse neurological disorders. However …

Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy

S Da Mesquita, Z Papadopoulos, T Dykstra, L Brase… - Nature, 2021 - nature.com
Alzheimer's disease (AD) is the most prevalent cause of dementia. Although there is no
effective treatment for AD, passive immunotherapy with monoclonal antibodies against …

Multivariable association discovery in population-scale meta-omics studies

H Mallick, A Rahnavard, LJ McIver, S Ma… - PLoS computational …, 2021 - journals.plos.org
It is challenging to associate features such as human health outcomes, diet, environmental
conditions, or other metadata to microbial community measurements, due in part to their …

Epigenetic encoding, heritability and plasticity of glioma transcriptional cell states

R Chaligne, F Gaiti, D Silverbush, JS Schiffman… - Nature …, 2021 - nature.com
Single-cell RNA sequencing has revealed extensive transcriptional cell state diversity in
cancer, often observed independently of genetic heterogeneity, raising the central question …

A decade of advances in transposon-insertion sequencing

AK Cain, L Barquist, AL Goodman, IT Paulsen… - Nature Reviews …, 2020 - nature.com
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Rapid non-uniform adaptation to conformation-specific KRAS (G12C) inhibition

JY Xue, Y Zhao, J Aronowitz, TT Mai, A Vides, B Qeriqi… - Nature, 2020 - nature.com
KRAS GTPases are activated in one-third of cancers, and KRAS (G12C) is one of the most
common activating alterations in lung adenocarcinoma,. KRAS (G12C) inhibitors, are in …