Outer membrane biogenesis

A Konovalova, DE Kahne… - Annual review of …, 2017 - annualreviews.org
The hallmark of gram-negative bacteria and organelles such as mitochondria and
chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the …

Outer membrane protein biogenesis in Gram-negative bacteria

SE Rollauer, MA Sooreshjani… - … Transactions of the …, 2015 - royalsocietypublishing.org
Gram-negative bacteria contain a double membrane which serves for both protection and for
providing nutrients for viability. The outermost of these membranes is called the outer …

Structural insight into the biogenesis of β-barrel membrane proteins

N Noinaj, AJ Kuszak, JC Gumbart, P Lukacik, H Chang… - Nature, 2013 - nature.com
Abstract β-barrel membrane proteins are essential for nutrient import, signalling, motility and
survival. In Gram-negative bacteria, the β-barrel assembly machinery (BAM) complex is …

Structural basis of outer membrane protein insertion by the BAM complex

Y Gu, H Li, H Dong, Y Zeng, Z Zhang, NG Paterson… - Nature, 2016 - nature.com
All Gram-negative bacteria, mitochondria and chloroplasts have outer membrane proteins
(OMPs) that perform many fundamental biological processes. The OMPs in Gram-negative …

Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry

DS Chorev, LA Baker, D Wu, V Beilsten-Edmands… - Science, 2018 - science.org
Membrane proteins reside in lipid bilayers and are typically extracted from this environment
for study, which often compromises their integrity. In this work, we ejected intact assemblies …

The structure of the β-barrel assembly machinery complex

J Bakelar, SK Buchanan, N Noinaj - Science, 2016 - science.org
β-Barrel outer membrane proteins (OMPs) are found in the outer membranes of Gram-
negative bacteria and are essential for nutrient import, signaling, and adhesion. A 200 …

The β-barrel assembly machinery in motion

N Noinaj, JC Gumbart, SK Buchanan - Nature Reviews Microbiology, 2017 - nature.com
In Gram-negative bacteria, the biogenesis of β-barrel outer membrane proteins (OMPs) is
mediated by the β-barrel assembly machinery (BAM) complex. During the past decade …

Monoclonal antibody targeting the β-barrel assembly machine of Escherichia coli is bactericidal

KM Storek, MR Auerbach, H Shi… - Proceedings of the …, 2018 - National Acad Sciences
The folding and insertion of integral β-barrel membrane proteins into the outer membrane of
Gram-negative bacteria is required for viability and bacterial pathogenesis. Unfortunately …

Lateral opening in the intact β-barrel assembly machinery captured by cryo-EM

MG Iadanza, AJ Higgins, B Schiffrin… - Nature …, 2016 - nature.com
The β-barrel assembly machinery (BAM) is a∼ 203 kDa complex of five proteins (BamA–E),
which is essential for viability in E. coli. BAM promotes the folding and insertion of β-barrel …

Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins

L Han, J Zheng, Y Wang, X Yang, Y Liu… - Nature structural & …, 2016 - nature.com
In Gram-negative bacteria, the assembly of β-barrel outer-membrane proteins (OMPs)
requires the β-barrel–assembly machinery (BAM) complex. We determined the crystal …