Advancements in next-generation sequencing

SE Levy, RM Myers - Annual review of genomics and human …, 2016 - annualreviews.org
The term next-generation sequencing is almost a decade old, but it remains the colloquial
way to describe highly parallel or high-output sequencing methods that produce data at or …

Next-generation sequencing—an overview of the history, tools, and “Omic” applications

JK Kulski - Next generation sequencing-advances, applications …, 2016 - books.google.com
Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation
sequencing have impacted enormously on the life sciences. NGS is the choice for large …

Genetic mechanisms of critical illness in COVID-19

E Pairo-Castineira, S Clohisey, L Klaric, AD Bretherick… - Nature, 2021 - nature.com
Host-mediated lung inflammation is present, and drives mortality, in the critical illness
caused by coronavirus disease 2019 (COVID-19). Host genetic variants associated with …

Prevalence and architecture of de novo mutations in developmental disorders

Nature, 2017 - nature.com
The genomes of individuals with severe, undiagnosed developmental disorders are
enriched in damaging de novo mutations (DNMs) in developmentally important genes. Here …

Comprehensive evaluation and characterisation of short read general-purpose structural variant calling software

DL Cameron, L Di Stefano, AT Papenfuss - Nature communications, 2019 - nature.com
In recent years, many software packages for identifying structural variants (SVs) using whole-
genome sequencing data have been released. When published, a new method is commonly …

Whole-genome sequencing is more powerful than whole-exome sequencing for detecting exome variants

A Belkadi, A Bolze, Y Itan, A Cobat… - Proceedings of the …, 2015 - National Acad Sciences
We compared whole-exome sequencing (WES) and whole-genome sequencing (WGS) in
six unrelated individuals. In the regions targeted by WES capture (81.5% of the consensus …

[HTML][HTML] A randomized, controlled trial of the analytic and diagnostic performance of singleton and trio, rapid genome and exome sequencing in ill infants

SF Kingsmore, JA Cakici, MM Clark… - The American Journal of …, 2019 - cell.com
The second Newborn Sequencing in Genomic Medicine and Public Health study was a
randomized, controlled trial of the effectiveness of rapid whole-genome or-exome …

The genetic structure of marijuana and hemp

J Sawler, JM Stout, KM Gardner, D Hudson, J Vidmar… - PloS one, 2015 - journals.plos.org
Despite its cultivation as a source of food, fibre and medicine, and its global status as the
most used illicit drug, the genus Cannabis has an inconclusive taxonomic organization and …

Primary immunodeficiency diseases: genomic approaches delineate heterogeneous Mendelian disorders

A Stray-Pedersen, HS Sorte, P Samarakoon… - Journal of Allergy and …, 2017 - Elsevier
Background Primary immunodeficiency diseases (PIDDs) are clinically and genetically
heterogeneous disorders thus far associated with mutations in more than 300 genes. The …

Genome sequencing of autism-affected families reveals disruption of putative noncoding regulatory DNA

TN Turner, F Hormozdiari, MH Duyzend… - The American Journal of …, 2016 - cell.com
We performed whole-genome sequencing (WGS) of 208 genomes from 53 families affected
by simplex autism. For the majority of these families, no copy-number variant (CNV) or …