All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

CRISPR-enabled screening is a powerful tool for the discovery of genes that control T cell
function and has nominated candidate targets for immunotherapies,,,,–. However, new …

CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …

Drug resistance is a principal limitation to the long-term efficacy of cancer therapies. Cancer
genome sequencing can retrospectively delineate the genetic basis of drug resistance, but …

The success of CRISPR-mediated gene perturbation studies is highly dependent on the
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …

Mutational scanning connects genetic variants to phenotype, enabling the interrogation of
protein functions, interactions and variant pathogenicity. However, current methodologies …

DNA methylation is critical for regulating gene expression, necessitating its accurate
placement by enzymes such as the DNA methyltransferase DNMT3A. Dysregulation of this …

CRISPR base editing screens enable analysis of disease-associated variants at scale;
however, variable efficiency and precision confounds the assessment of variant-induced …

A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …