DNA-loo** mechanisms are part of networks that regulate all aspects of DNA metabolism,
including transcription, replication, and recombination. DNA loo** is involved in regulation …

The mechanical properties of DNA play a critical role in many biological functions. For
example, DNA packing in viruses involves confining the viral genome in a volume (the viral …

Abstract Effects of placing a lac operator at different positions relative to a promoter for
bacteriophage T7 RNA polymerase were tested. Transcription can be strongly repressed by …

Transcription factors (TFs) are proteins that regulate the expression of genes by binding
sequence-specific sites on the chromosome. It has been proposed that to find these sites …

We directly observed real-time production of single protein molecules in individual
Escherichia coli cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) …

We have constructed a large library of random peptides fused to the C terminus of the lac
repressor. The DNA binding activity of the repressor protein physically links the peptides to …

In gene regulatory systems in which proteins bind to multiple sites on a DNA molecule, the
characterization of chemical mechanisms and single-step reaction rates is difficult because …

We present a quantitative case study of transcriptional regulation in which we carry out a
systematic dialogue between theory and measurement for an important and ubiquitous …

A transcriptional cAMP-responsive enhancer element (CRE) consisting of the 8-base pair
(bp) palindrome, 5′ TGACGTCA 3′, is found in several eukaryotic genes. We analyzed …

Biomolecular assembly is a key driving force in nearly all life processes, providing structure,
information storage, and communication within cells and at the whole organism level. These …